FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WRKY40_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WRKY40_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216646
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC9000.015.16673870
GCGTTGC2600.001596762910.7692870
CCCCGGG2650.001871328510.56608770
GGCATCG4158.029048E-610.12052970
CGGAAGC13000.09.69235370
CACTTAG27400.08.94164870
AGGCATG9403.6379788E-128.93621270
CGTCTGC5906.8333975E-78.89834670
CCGTCTG4451.8939956E-48.65172568
AAGAGCC15400.08.4091370
GCCCAGG15550.08.3281421
GTCACTT38700.08.13957270
TAGGCAT13350.08.12737868
CACACGG3900.00485710478.076961570
CAGTCAC45650.08.05042169
TTAGGCC5301.4258141E-47.924565370
TCACTTA37350.07.77781470
GGAGGGC4955.9637264E-47.77781467
GGCATGG7805.723814E-77.62824170
GCGGAAC5057.387029E-47.62367932
Produced by FastQC (version 0.11.5)