FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WRKY40_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WRKY40_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCTTGATAACTTAAAATATATTTTTCAAAAAATTAACTCTTACTTA11.282051282051282No Hit
TTTCATTCTAATAAATTTCATTCTAATAATATAATAACTAATAATAAAGT11.282051282051282No Hit
TCTATTAATGGACTCATCCATGAGAAAGTCAAAAGACTGAGAATGCCAGA11.282051282051282No Hit
AGACTTCATCGACCGGGTCCGAGGAATCGTCGACCCGGACGATGAATGGT11.282051282051282No Hit
ATCCGGGTCGGGTTTTAAGAACAGGTCAATTCAAGACCATTGAGGCTTTG11.282051282051282No Hit
GAATGTAGTATACCTAAGAGAGAAGAAAGGACTCGACGTAGTTAAAGTAG11.282051282051282No Hit
CGCGTAAGTAGTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATAT11.282051282051282No Hit
ACTTTGCATAGAGCTTTAACAAATGCTACTATGGCTTGAGTCTTCAGCCG11.282051282051282No Hit
TGAAGAAAGGTCCTTGGACGCCTGAAGAAGATGAGGTTCTTGGTGCGCAT11.282051282051282No Hit
ATTATATTCGTGACCTCGGAAGTCAAGGCCTGTCTTGGTGGAAGAATGGC11.282051282051282No Hit
AGTACTTAAAGGTCAAAATTTGGAGTCGACAAAAAGTCAATGGAAAAGTT11.282051282051282No Hit
GATAAAGGTCCGAATAATTCTCTTGTTAAGACTGAAACAAATTTGGTCTT11.282051282051282No Hit
AAGGTCAAACTCATGACGAAGTCTGAAACCCTACTTCTTTGTTGACTATA11.282051282051282No Hit
TTACAGAATTTGAAGACCTTCGACTTCGTCTTGAAGTAGAAGCGTCAAGA11.282051282051282No Hit
CATTGGGCATCGAAAACGAGAAGTAAGTCGAGAAAATAACGGAGTTCCTC11.282051282051282No Hit
TGTAGACAAAGTCAATATGAGTCTTTGGCTTTGTGTCTTTTTACAAGGGT11.282051282051282No Hit
GGAATGGGAGGACTAAAGAGAAGAATTTCGATTGAGAAGATTCAGAAGAA11.282051282051282No Hit
CATGTGTATGATTGTGTATAACAACTTAAACAGCAACCCGATCTTAAAAG11.282051282051282No Hit
GGGACCGAGAATTCGTCGACCACGCCGGCGGAATGCTCGCCCAGGACGAT11.282051282051282No Hit
TTACTTATGACTATATGAGGTTCATTTCCCTTGAACCACACATGCACATG11.282051282051282No Hit
GATTGGTCGAAGGGAAGATATACGTGACGGGACTAGGTCAATGAGAGAAC11.282051282051282No Hit
TAAACATGTGCAAGACCTTATCTATATAAGTTTCTTGACTTAGTCCAATT11.282051282051282No Hit
GATTAGCCGAGAGTACGGTGAGATTTTAAAAGTTCTCGATGATTTCTTCA11.282051282051282No Hit
ATCCAGAGGTTTAAGAACATTGTCAAGGACGATAAGAAGTCTTTTTTGTC11.282051282051282No Hit
CATGTCGTAGTTATATACGGCGTGGTAACAATAGGCTACACGAATGGGTT11.282051282051282No Hit
AAGTAAGAGAAATAGAAAATCTCATCTAATTACCCAAATAAACTTGTTTT11.282051282051282No Hit
GGAGTTTTCCGTATAGCAGAACGATAGAAATATGGTTGACTTTGATTTCT11.282051282051282No Hit
TTTGTAACCCTTATTTACGTGATAAAAAAGTTTAGCCTATCGTTCCACTA11.282051282051282No Hit
TTCCATCTTTCAATATAGAAGACCCACGGATGTCGCGGTCAACGGCCACC11.282051282051282No Hit
ACGGTAAAGTAAACTACTAAATTATATATTTTGCTCAACATTGAAATGAT11.282051282051282No Hit
AAAAAAATCATTGGCTTATCAAATTATCTATCGCGCCTTGAAAAAGATTC11.282051282051282No Hit
GATAAATGGGAAATTCAAAAAAGATGCTTGGGGATGAAAAAGAGGGAACA11.282051282051282No Hit
TAAGAGAAGAAGTCTTCATGGCTATTACCATCATCATCATCAGTTGCATT11.282051282051282No Hit
ATTATACCTCTTGTGAATCAATTTTATGACTTTCAAGATCGGAAGAGCAC11.282051282051282No Hit
TATTAAGAAATGGTCCAACCTTTTCAAGTTTGACACCCATAAATTGTCAT11.282051282051282No Hit
AAGTTATATTTTAACTTTTGTATATAATATAATTGTGTATTGGTAAACAG11.282051282051282No Hit
TGATTGGGTAAATACGGTCTTAGACAAACAAACTGCTGGCTTATGTATTG11.282051282051282No Hit
GAGGTCAAACGCAGCATCAACATTTATAAGAACAAACCCTCAACAAATAT11.282051282051282No Hit
TACATTATATGTTTTCAATATCTTTTCAAACAGGACGTGCGTAGAAGTAC11.282051282051282No Hit
ATCGAGACAAGACTGGTCATGAAATAGATGATGCTCCAACAGGAATAGAA11.282051282051282No Hit
AAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTAGATC11.282051282051282No Hit
AGGGAAGTCCGTCAACGTGCACACTAGTTCATATATTACATAGCATGTAA11.282051282051282No Hit
TATTCTTTATCTTAATGATTTGGTCAATCATGAATAATTGGGTTCTGAGA11.282051282051282No Hit
CATCAAGTGGGTGGAGAATCCAGCAATCTTGTTGCGGAGTCTCTTTGAAG11.282051282051282No Hit
CTGATGCTGGGAATGTTGGATTCAACTAAACACGACCATCAATGATTCTT11.282051282051282No Hit
GGTAAAGGTAAGGATTTGATACTTGTTTCTTTCTTCAATACTGAGATGTA11.282051282051282No Hit
GTTAGAAGACACAAAGCCAAAGACTGATATTGACTTTGGCTACACCATGA11.282051282051282No Hit
AAAGCAGCAAGACAATGGAACTTTTCCATTGCCTTTTTGTCGACTCCAAA11.282051282051282No Hit
TGTAAGGGAATGATTTAATGGTTTGTTTTGACTTGTAGATATATATTTCT11.282051282051282No Hit
AATTAATTTGTGAATTGAACTTAACTATTTGAATTTTTTATATTGATCTT11.282051282051282No Hit
CGAGCCTCATCATCTTAGATTCAAGAGATATTCTTAGTCACGTCTTGAAT11.282051282051282No Hit
GAAGATGACACAAAGCCAAAGACTCATATGGACTTTGGCTAAACCATGAA11.282051282051282No Hit
TTCCACCATTTCATTTTATCAAGTTCAATGATAGCCAGATTAGTCAACAT11.282051282051282No Hit
CTCCTTTTCCTATAAAATAAAATTTCACCCCATTAGGAATAGTTTAGTAA11.282051282051282No Hit
CAGAGAATCGAAGTCAAAGCAGCTTCTTTTGTGGTTAGGTAAGAAAATAT11.282051282051282No Hit
GTATGTGGTACAATTGAGACTTGACCACTGTACCAGAATTGAAGTAGAGC11.282051282051282No Hit
CCATTACCATTTCATGTTGAGTGTTGACTTTGTGATAATATAGTGATAGA11.282051282051282No Hit
ATGAAGACTCACAATGAATAGTCAAATAATAACTCCTAGGGAAAATACAT11.282051282051282No Hit
TCATCGTCCTGGTCGAGCATTCCGCCGTCCTGGTCGACGAATTCTCGGTC11.282051282051282No Hit
TATCGGGTCCGAGGAATCGTCGACCAGGACGAGGACTCGTCGACCGGGTC11.282051282051282No Hit
CCCTCTTGGCACCAACAGCCAGTGAATTGTCTGAGGCGAGCCCGGTGCCA11.282051282051282No Hit
AAATAGTTACTAGATTTTATTCTAATCAAACTATATCCATCACTCAAATA11.282051282051282No Hit
GCAGAGAATGCTTGGGGTTACACTCACGAAGGTGCATAGTGAGAAGAGTA11.282051282051282No Hit
TATGTGAGATTTTCCTCTCATACGGCTCCTCCGTTAGGTGCATAATGAAA11.282051282051282No Hit
TAAATAAACTTATAAAATTTGGGAAAACGGTCGGAGAGCATTTATATCAA11.282051282051282No Hit
ACATGATGTTTGATGAAGTATGCCGTGTGTTTGAAAGTAGGGTGTTAAAC11.282051282051282No Hit
TGTCACCACCAAAAGTAAAGGACTTAATGAAGACTATCTTCTGAAATCTT11.282051282051282No Hit
TATTTTGTCCAGTCATACTCAACTAATTAACCAATGCATGTCTCTACATC11.282051282051282No Hit
CAAGTCTCCTAAAAAGACCAAAAAGTCTCCTTAGATATTTATCAAACTCT11.282051282051282No Hit
ATCCGGTCAAATGACCTAGCTAGAGGTGTCAAAAAATTATGAAAATTTAC11.282051282051282No Hit
CTCTCTTGACTTGGGTTACAGGAGGTTGAACGAAGTACAAACACTCTCGT11.282051282051282No Hit
CTTGTGAAACCTACATTCTTTTTGACCCATTACGAAATCAATCATATAAG11.282051282051282No Hit
GACACTCCTATGGAGAGAGTTGACCAACTTTCATATATAAGATTTCAGCT11.282051282051282No Hit
ACCTTGGTTTTGGAAAAAAAGTTATGGACTAAGCCAACTACGTTCATTAA11.282051282051282No Hit
CATAACGTCAAGTTTGACTTGTTATCACTAGACGCGGAATCTGAAACTAG11.282051282051282No Hit
TCCATGATGGGTTTACCAATAGACATAGCTTTGAATTTAGAGGAAGAAAA11.282051282051282No Hit
GTGAAATGTCAATTCTACTTAAATAACATTGCACCAATAAATCAAGTCCC11.282051282051282No Hit
TTATGGCATTACCAAACCAAGCAATACCAGCAGTGTTGACCCAAAATAAT11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACT50.070.055
TTTGAAT50.070.028
TGTGAAT50.070.09
TATATTG50.070.039
ATCTTGA50.070.046
TGACCTT50.070.050
AATTTTT50.070.032
AATTTGT50.070.05
TGACTAT50.070.057
AATTTGA50.070.065
TGATCTT50.070.044
TTTTTTA50.070.034
TGATCCA50.070.069
TTTTTAT50.070.035
AATTAAT50.070.01
TTATATT50.070.038
TATTGAT50.070.041
TTTTATA50.070.036
AACTTAA50.070.018
TAACTAT50.070.022
Produced by FastQC (version 0.11.5)