FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WRKY40_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WRKY40_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5778945
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC14050.012.70456370
AAGAGCC28550.010.29767370
GCCCAGG23650.010.2115431
GTCACGC8450.09.9407870
CGATCAG33000.09.43934870
CGGAAGC22750.09.38456870
CCAGTCC12450.08.99593970
GGGGCGG3550.00207584038.87342659
CACGATC45350.08.64383870
CACACGG7151.425069E-78.32163770
CCCAGGG5906.5359236E-68.3050442
CAGTCAC81050.08.11840570
AGTCACG73100.07.85221870
ACGATCA45050.07.84679870
GCACACC8503.4129698E-87.82349170
TATGCCG4500.00201379277.77787446
CTCCAGT113150.07.733059470
GTCACGA60150.07.44800970
TGGGGGG4700.00308497237.44683655
TCACGAT54650.07.429057670
Produced by FastQC (version 0.11.5)