FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WRKY40_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WRKY40_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5778945
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC13950.012.54474170
GTCACGC8600.010.58134370
AAGAGCC28600.010.2796770
GCCCAGG23500.09.9788471
CGATCAG32850.09.37590570
CGGAAGC22700.09.25105670
GGGGCGG3500.00182467139.0001959
CCAGTCC12300.08.821094570
CACGATC45350.08.79819370
GCACACC8554.838512E-108.59644970
CACACGG7101.2729834E-78.3802470
CCCAGGG5906.5359236E-68.3050442
CAGTCAC80200.08.20444870
CTCCAGT112100.07.805492470
ACGATCA44800.07.73433770
AGTCACG72350.07.691737770
TGGGGGG4650.00277837727.52690945
TCACGAT54700.07.42226769
ACTCCAG120200.07.308615769
GTCACGA60150.07.273446670
Produced by FastQC (version 0.11.5)