FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WRKY40_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WRKY40_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAGGTCAAAATTTGGAGTCGACAAAAAGTCAATGGAAAAGTTACA12.3255813953488373No Hit
CTATTGTTATTTTCATTTTATACCCTTTTTTTAGTTGACGGGTTGAAGCA12.3255813953488373No Hit
GGTAACAACTCAAATCAAGACAAAGATTCCCGACGAGGGTCATGTCTTTG12.3255813953488373No Hit
CTTACAAGAACAGAGGAGTCTCTAGCGGCTAGGGTAAGAACATCAGCGCA12.3255813953488373No Hit
GTTTAAAACAAACAACAAAATTGCAAGAAATTAAACACTAGTTATCAACT12.3255813953488373No Hit
CTTTTCATTTCTACCTCAGGGTCTCTGTCTTGTTGACTGGGGGAGAGGAA12.3255813953488373No Hit
ATATATAAAGAAATTGTTTTCTCATAATATTACAAGAACTTAAAATATAC12.3255813953488373No Hit
CATGGGTACCTGTTTACGATGATGATGACTTTTGCCTCCGGTTTAAGTGG12.3255813953488373No Hit
GTTGTGGATTAAGATTTGACCGTATCTATTTTTATGGGCAGTTTTAAATT12.3255813953488373No Hit
GATGATATTGACTAAGAAAAGAAAAAGAAATTCAAAGTCCGACCTGAATT12.3255813953488373No Hit
AAAATCATATTCTTCTTAGAATGTTTCTGCCTGTATAAGGAAGACTAGAA12.3255813953488373No Hit
GACAAAGCCTCATATGGACGTCGGCTAAACCATCAAAGCTTTGAGAAGCA12.3255813953488373No Hit
CCAAATAATACTTTCCTCCTTGACCATCGCCAGAAGCTACATAGGATGGC12.3255813953488373No Hit
CTGATTTGTGTTTATGATGCCAGGTCAAGGATCTTGAAGCTGCTGTACAA12.3255813953488373No Hit
GTCATTCAACCTATATTCAGGTGCAGATGTCTGATTTGCCCGGTGAGTTC12.3255813953488373No Hit
TAACAATTTCAACAATCAAATCAATCTACAGAGATAAACCATCGAAAGAC12.3255813953488373No Hit
GGTCAATCACGTAAGATTGGTGAATTTGTTGAAAGTACGGAAAGAGATGT12.3255813953488373No Hit
TTATCTTTTTTCAGTCTGAAAAATAGAGAGAAGCAAGTCTATTAAGTTCA12.3255813953488373No Hit
TGCTGTCTTCTTATTAAAGGGACGGGAATTGACATAATTTTTCTTTTCAA12.3255813953488373No Hit
ACCTCAGCAGGTGATCCGGCAGGGTCGGTCAAAAGAATGCGAGAAGAGCC12.3255813953488373No Hit
ATTTGGATAACATACAAAAGACAAAAACATTGCCCTAGTGTTTTCCAATT12.3255813953488373No Hit
GAAAATTCCAGAGTAATACAACTGGCACATTGTGTAAATTTTCTTGATAT12.3255813953488373No Hit
CGGCCACGGTCGCTGAAGCCTCGGCCCTGGGCGCCGCTTTCAACCCCTGG12.3255813953488373No Hit
GATTCTTGCGTAAACAATACAAAACATGTGGACGACCAAAGCCTTTTCCA12.3255813953488373No Hit
TGGCATGTGATACCTTTTCGGAAAGCCCAAAGAGAGCCCTCCGACGAAAG12.3255813953488373No Hit
TGTTATGACTCATATATTTTTGACGTAAGTCAGCCGCAACATACCGTAAC12.3255813953488373No Hit
CTGCTGGAAGATTACTTTACATGGGGACAAGTTTATTTCAAGAGACAATA12.3255813953488373No Hit
GGAAAAGTTCCATTGTCTTGCTTCTTTCGTCGGAGGGCTCTCTTTGGGCG12.3255813953488373No Hit
GGGATCGCCGGATTTCTCTTTCAGCATTTGCTCTAAATTGAGTAGGGATT12.3255813953488373No Hit
TTTAATAGAGCGGTCTAGACGTAATTTTTTATCCGTAATCCTAGCGGACT12.3255813953488373No Hit
AGGCATAACATATAAAATAAAATAGTTTACGCATTTTACATCATTCAAAA12.3255813953488373No Hit
TTCAAACGAAATAAAAAGTCAATACAATTAGTCTTGTAAGAGCGATGATC12.3255813953488373No Hit
CTGCTTACAGAATTCAGTTACACCTTAGACAGGTCCAATGAACAGACATT12.3255813953488373No Hit
CCATCTAATTCCCAGCCCCTGTACCCCAAGATGAAACAAGCCCTTATGGG12.3255813953488373No Hit
GTGCAAAGAGCTGGCATGTAGCTGACGATGCACCCTTTAATGCATAAGTT12.3255813953488373No Hit
TCTATTGGAAGATTTGCTTGTCAAGTAATCTTCGCGCGGTCTCTCTGTAT12.3255813953488373No Hit
ACTCATTCATGTACTATAACATGATAAATCGACCATTCTTTATATTTTTT12.3255813953488373No Hit
GCACCCAACTTTATTTTCTGACAAAATTTACCAAGTCTACGCGGGCCCGC12.3255813953488373No Hit
CACTTGAAAAATGTTATTACATATTAAGATATTAATTTGTATGTGTGTAC12.3255813953488373No Hit
GCAGAACTTGTACTCCATGATCCCTAGCCCTGTAGATAGAGAGATGGGCA12.3255813953488373No Hit
TCGGTTCTTGCCTTCTTGGTATAGAGGGATTATTCAACTAGTTAGGATCG12.3255813953488373No Hit
CTACGGATCAAGTAAGTCGGCAGGTTAATCACGTTTAGTTGCCTAATTTA12.3255813953488373No Hit
GTGTTTCGTCGTTTGAAGATACAAAGTCAAAGCCTCATACGGACTTCGGC12.3255813953488373No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)