FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WOX11_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WOX11_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267284
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC3501.0409658E-611.99996570
CATCACG8352.8085196E-98.38320770
CAGTCAC22900.08.2532570
AAGAGCC8151.6165359E-88.15948570
CCAGTCA24300.07.34565769
ACATCAC19700.06.928912670
TCCAGTC26050.06.7178369
ACTCCAG30800.06.59088869
CTCCAGT28650.06.474674770
GTCTGAA54100.06.14600769
GAACTCC36000.06.124981470
GTCACAT23450.06.11938469
TCTGAAC50600.06.086937470
CACGATC6400.0089130696.015606469
TCACGAT8751.7496277E-45.99998167
CTGAACT47750.05.93715570
CGTCTGA54750.05.817333768
TCACATC24200.05.640478670
ACGTCTG59900.05.492470767
AGTCACA27500.05.4727169
Produced by FastQC (version 0.11.5)