FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WOX11_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WOX11_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGCATTAACTAGAGACCCAGCGTAATTGACCTCATATCCTTCATTAT15.0No Hit
ATATAAGTGTTGTATTCACTTTTCTATGCAAATATTAAATGTATTTAATA15.0No Hit
GGTTACTTCCAATTACGTAAATCAACTAGTTCAAACCGCACTCAAAGGTA15.0No Hit
ATTAATTATAAAACAGTAATTAACTAATTAAGTAATGTAAAATGATGATA15.0No Hit
TAGTAATTTAAAAATATATATAGTCAGCTTTGTTAAACTATTTATATATG15.0No Hit
AATAAACTTATCATAAATTAAAATATGGATAATAATTTACTTCTAACCCA15.0No Hit
CATTTTTGACCGAGAATAGTGGTCGAAAACATCTTACCAAAAAGTGGTCA15.0No Hit
CCTTGAGGATTTTGTGAGGTATTGTTGATGTTTCAAAGTTTTAGATTTCT15.0No Hit
ACTTTAATCTTAACCAAAGTTTGTTGTTAGAGTTTGCAAAATGAAACAAC15.0No Hit
GTTTCTCTCGTTTAGTTTATTCAGTTCTTTCGTTATTCCCCTGTAAATTC15.0No Hit
ACATGTACCATATACGTTTTTTATAGTTATCATGTACGTTGGGGTGTCTA15.0No Hit
TATCATGATGATGATGGCGTTTGTTGATTCCGGAAATTACTTAATCAAAG15.0No Hit
TAAGCGAAAATGTCAATCAAGCTTAACTAATATGTAAGAAAGTAATTGAA15.0No Hit
GTTCCCTCTCTCTCTTTCTCAATTTCTGTCTGCGTCGATAGGATTTATGA15.0No Hit
TACATTATTATACTAATAAATTTTGAATTTTTTTGAAATTAAAGTTCTTG15.0No Hit
ATTATTATTGAATACAGAGTTTGAAATATATACACATACAACTATTGCTA15.0No Hit
TATTAATTCACCTACTATATCTCAACCTTTTATTTCCTCACTTATGAGTT15.0No Hit
TTTCTATTCAACCCAAAAAATAAAATTAATCATCTTACTATATAATTATC15.0No Hit
ACCTTCTCTATCAAATTTTTCAAAAAATTACAATTTATCAACATCAAGTA15.0No Hit
GTTAGTAAATAACAGCTACTGGTTTGCTCTCTCTGCCGATGAATAATGTG15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)