FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WOX11_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WOX11_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6073322
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGC11950.013.18007170
CCAGTCC5801.8189894E-1212.06910870
CACGTCG4451.105218E-811.79789170
AAGAGCC19700.010.12702370
GAGCACC9900.09.89910670
AGGGGGG4351.4051091E-59.6552065
TGGGGGG4005.930929E-59.6250335
AGTCACC33150.09.60795670
TAGGGGG3350.00122409079.4030194
ACTCCAG57300.08.55158470
CAGTCAC38450.08.01043269
TCACCAG27750.07.56759469
CCAGTCA43050.07.56100268
CTCCAGT51650.07.5218770
GAACTCC71100.07.38405370
CACCAGA28150.06.962781470
GTCACCA30950.06.898247268
TCCAGTC47550.06.771898770
ACGTCTG117850.06.058620570
TCTGAAC102250.06.02452170