FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WOX11_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WOX11_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6073322
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGC11950.013.47296170
CCAGTCC5801.8189894E-1212.06910870
CACGTCG4409.3496055E-911.93195870
GAGCACC9900.010.60618570
AAGAGCC19550.010.20472370
TGGGGGG3955.1672123E-59.746875
AGTCACC33100.09.72821170
AGGGGGG4401.6762826E-48.75003055
ACTCCAG56700.08.3951670
CAGTCAC38500.08.09093869
CCAGTCA42850.07.75965468
TCACCAG27800.07.679882569
CTCCAGT51500.07.54377870
GAACTCC70500.07.44689670
CACCAGA28100.07.09972770
GTCACCA30700.07.06842968
TCCAGTC47500.06.85271267
ACGTCTG116450.06.131458870
TCTGAAC101500.05.89662170
AACTCCA88750.05.83664169
Produced by FastQC (version 0.11.5)