FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WOX11_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WOX11_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACCCAATTATAATTTTACATTTTTTTCCATCGCAAAACTCTTAAA11.9230769230769231No Hit
CTACAATTAATGATAAGTACACGTGTCATCAAAGTAGAGTGCCAAATGTC11.9230769230769231No Hit
TCCGGATTCTCCACCCGACCCGAGTGATACGTACGCCTTCACGGTCGTTT11.9230769230769231No Hit
CTCAAGCAGAGAACTAAGAAAAGGACACGAATGCTCAGTGTTAACGACGG11.9230769230769231No Hit
CATACCAAGTTCTTCTTCCTACCATACATGGTTTAATATTGTTTTGGGAA11.9230769230769231No Hit
TTTAATGTACCTAAATTTTGATTTAAATTGTATTCCTTGAAGTACGCATA11.9230769230769231No Hit
TTTTCACAGAAGTGGACGAAAAAAGATTTTCTCTCACCGTTTGTCAAAGT11.9230769230769231No Hit
GCCTTTTCTTAGTATTTTTTTGTTAAGTATAGTTATGATCTTTTCAATCT11.9230769230769231No Hit
TACCAAAATAGTATAGGTGAATGTTATAAAATCGAAAGTACGAATACTCA11.9230769230769231No Hit
ACCAAAGCTTTTGTCATTTAATATTTGTTTAGATGTGCTTTGCTGCTTTC11.9230769230769231No Hit
GTCCAATCTAAAACTGACAAAGAGGTTAGTTAGTAAAGAAAGAAAGAAAG11.9230769230769231No Hit
TTGAAGAGCTTACATTAATATATTTAATAGACACATGTCAAATGCTAAAA11.9230769230769231No Hit
CGGTCTGAGTATACGCCGTCAGCCACGTTCGGCCGACAATTCATGTCCCA11.9230769230769231No Hit
TGGGTGAATTGCAAAACTAACTGTTTTTGATAATTTATTTAAGAAACTCA11.9230769230769231No Hit
GTGTATTTTATTAATATATATATTAATTATCAAATTTGACAAATTAATAA11.9230769230769231No Hit
AAATGTTAAAAAAAATAAATAAATTAGGATACAGAATATAACAACTGATG11.9230769230769231No Hit
TCATCATTTTAATTTTCTAAAAAAAAAAAATTCAAAGCCCAAAAATTTCT11.9230769230769231No Hit
GGAGGAAATTAGTCCAGTGGTCATGGTTCTATGTACGCCTCTTGTTGATG11.9230769230769231No Hit
GCTTCACCTTCTTTCCTTCACTTTTAGTTAGCGGAACAAATTTCTTCAAT11.9230769230769231No Hit
GTAAGAAAACTGACTTTCTGTGTGTTTGAAAATAAGTCTGCTTTACAAAT11.9230769230769231No Hit
TTGTATACCTGGTTTCAATATATATTACTTCGATATGTAAATCATGGGAT11.9230769230769231No Hit
TTTTCCTGTAACTGGTCTCTTCATAAGTTTTTCCGTGATGAGTTCAGGCA11.9230769230769231No Hit
CTATTATCTTAATTACAAATAACTTTCTGAAACCTTTTTTTTTATTGGTA11.9230769230769231No Hit
GCCAAAATACCAGAGAGTAAACAAAACACAGTTTACAACCCAGTAGAAGA11.9230769230769231No Hit
TTATGGGTTAGGGTTGTGATGTGGGTTATTTTTGTTTTAGCATGTCGTGG11.9230769230769231No Hit
AGCATAGGTAATTGATACGATCTAGTAGTTTAGTGCATTTGTATGCATGC11.9230769230769231No Hit
TATCTTTTTGACTCTGAATTAATTCAGCAGAGATGAAGATGAATGTCTTC11.9230769230769231No Hit
CTGTGGCTACCCCATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTTAGA11.9230769230769231No Hit
TGTTTAATTAACGACCTTACTATCTCTTTAAGATGATATTCAATTAAGTA11.9230769230769231No Hit
ATATTATTTTTACTGAGATAATTACCCAATAGCACCTTTAAAACTTGAGG11.9230769230769231No Hit
CACCGTGGAGCAATAAGTTGTATTCTCAGCTAGCATTCAATGATGTAAAA11.9230769230769231No Hit
TTCCTTTCACACTCTTCTTTCATATGTTTTTGTGCATCATCAATCTCTTT11.9230769230769231No Hit
TGCTTTCCCAAAGAATGCTGAAACTTATCTCCATAGGGTAAGCTAAAAAC11.9230769230769231No Hit
TATTAATCCACACTCTTCACACTAATTCAATTTTATCCCTAATAAAAAAC11.9230769230769231No Hit
CTTCTCCATAATTTAGGTGTTGAGGAGTGTTTCTTGGCTATAACTTCATT11.9230769230769231No Hit
TATTAAAACTAATTTTCGCCACGTGTAGAAACGATATCACTTCTTTACAA11.9230769230769231No Hit
TACTGTTAACTTATTTTAGACTATTTTACCCTTTACAATTTCTTTTATTT11.9230769230769231No Hit
ATTAGTCTAAATTTCTAAGCCTATTTAAACCTCTTTATAAATTGTGGACA11.9230769230769231No Hit
CCCCACGAGTGTAGTGTGCAGGACCACTACCCCATAAAAGGATTATTAAC11.9230769230769231No Hit
GTATGACTACTTGGTGGTAGATATGAGAACCGACGAGATAATATACAAAA11.9230769230769231No Hit
TTATATATAGAGTTAATATATTCAAGACCTCTAATTACCATCCATTTTGT11.9230769230769231No Hit
GTTTGGCAAGCCGCTGTAAGTTTTCGATAAAATTCTTAATACTGTCTTAA11.9230769230769231No Hit
CCACACAAACTTTGTATCAAACAATATCAAAATCACTACTAGTTAGTGAG11.9230769230769231No Hit
GGCTATGCACCACACAGGCTTCCCCCCCCTAGTCACCACACTTCCCATCC11.9230769230769231No Hit
CATAACTATAGGACAAAGAATGTGGTACCTCTTAAACAAGAAGCAACACT11.9230769230769231No Hit
TCCGATGGAACCTTCTCTTGTACCGGGGATGGCCATTTATGCATGAACCA11.9230769230769231No Hit
CAATAGCGCGTAACGCGTTACAGCTAAACCCAATCAGAGTGAAGTTTCAA11.9230769230769231No Hit
CCCGTCCAAGTCACCGACATCATCATCAGTAGAATCCATTCAAAAGAAAC11.9230769230769231No Hit
GTGTACACATCTGCCTCTTCCCCACCCACCCTTCATTTGTTAATTAATTT11.9230769230769231No Hit
TCCACATCCTTACGGTTGAAAATCTACAATGGCTAGTCTTATTTGTAATA11.9230769230769231No Hit
CGCCCATCATCATCCAACTACAAGGTTCCCCCCACCCAATTCTCCCCAAG11.9230769230769231No Hit
TCCCGTTCTCAACCGATGACCAATATGAACTCGAAGTTTCCTTCGTAACC11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAG50.070.024
TTTGAGA50.070.022
TTTTGGA50.070.052
GAGAAGC50.070.025
TGGTTAG50.070.043
CAAGAAG50.070.031
GGTTGGT50.070.040
AGCTTTG50.070.019
CATGAAA50.070.013
GCCTTGG50.070.068
GCAAGAA50.070.030
AGAAGGT50.070.036
AGAAGCA50.070.026
AGAAGAA50.070.033
GTCGCAT50.070.059
CATCTGC50.070.063
GAAGCAA50.070.027
CCATGAA50.070.012
GTTTTGG50.070.051
ATGAAAG50.070.014
Produced by FastQC (version 0.11.5)