FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160234
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC6600.018.03043470
GAGCACC12600.015.27788870
ACAGTGC7450.014.56386470
CACACGG4509.804353E-1012.44453470
AGTCACC8400.012.08342170
CGGAAGC15650.011.629477570
CACGTCG4054.931753E-711.23464970
CAGTGAG11500.010.347970
CACAGTG45500.010.1539269
TGAGGGG3904.491159E-59.87186770
CAGTGAT30950.09.72543369
AAGAGCC18400.09.3207270
ACACAGT54350.08.56491970
CACACAG61300.08.45030570
CGTCTGC8302.5083864E-98.43379570
AGTGATG34300.08.36740870
ACAGTGA40700.08.25558868
GTCACAC78400.08.21434570
GTGATGG21850.08.16939570
CAGTCAC100300.07.781711670
Produced by FastQC (version 0.11.5)