FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160234
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC6800.018.01483770
GAGCACC12550.015.33875770
ACAGTGC7500.014.9334470
AGTCACC8350.012.15577670
CACACGG4357.885319E-912.06905370
CGGAAGC15650.011.629477570
CACGTCG4105.778911E-711.09764170
CAGTGAG11700.010.470160570
CACAGTG45950.010.1306569
TGAGGGG3853.8976304E-510.000072570
CAGTGAT30950.09.61234769
AAGAGCC18300.09.37165370
CACACAG61700.08.565701570
CGTCTGC8201.9990694E-98.53664770
ACACAGT54600.08.52570270
GTCACAC79050.08.2796370
AGTGATG34450.08.22937870
GTGATGG21800.08.18813270
ACAGTGA41050.08.18519968
CAGTCAC100800.07.708389370
Produced by FastQC (version 0.11.5)