FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGGAAGAATCAGTTCCGTAATCACACCTTTTTTGTTGCTTTGATGA14.761904761904762No Hit
TTTTTGTGCTTCCTGGATTCTCCACAATAAGACTGCCTGATTCACATCGT14.761904761904762No Hit
TTGCATTTAAGTTCAGTCCTAAATCCACAGTGCTTTCGTTCAAATCTTGT14.761904761904762No Hit
TTTAGAGTTGTGAAGCAGTTCATGGTAAACACAGTTTAGAAAATAATAGT14.761904761904762No Hit
CTATACGTGGCCTCTACCAGTGGTCGCGTGATAGATGTATCAATAAAGGT14.761904761904762No Hit
ATGCGCAATCCAGCTTTCAAAACTGTCGTTAAAGATTTCTAGCGCCGCTT14.761904761904762No Hit
CATTGCAGCGAACTTGTATATGACACACCACAAGATTCCCTTAACCGCAG14.761904761904762No Hit
GTTTTCCCAATCAAAATCGATTTCCTAAACGTAACGCCTTCACATTGCTT14.761904761904762No Hit
TGTTTGGGTTCACACATTTGACCGCAGAATCACTTTTAAATACATGCATT14.761904761904762No Hit
ATCTAAATGCATTAAAATAAACTAGGTTACTTCGATTAAATTATGAATAT14.761904761904762No Hit
CATATGGACTTTGGCTACACCATGAAAGGTTTGAGAAGCAAGAAGAAGGT14.761904761904762No Hit
GCAAGGGTCTTAACTCTTAAACCGGACATTCTAATTGAAAATATAAATAG14.761904761904762No Hit
GAGTACGTGTGAATAGAAAAAGAGAGCTTGAGAAAGATAGAAGGAAACAT14.761904761904762No Hit
ATCCGGTTGCGGTTTAAGTTCTTATACTCAATAATACACATGAGATCAAG14.761904761904762No Hit
AATGCAATAGTTTTGCTTAAACTTAAATCAAAGTATTATTCCTATTTTCG14.761904761904762No Hit
ATATAATGTCACCAAGTACAAAAGTGAAGAAATCACTTTTTCGAATTAGG14.761904761904762No Hit
TTACTTTGCTAGAGAAGAGAAGCTTCTTGTTTATGATTACAAGCCTAATG14.761904761904762No Hit
CATGATTGAGACCATATGTTACATTCGTCTAAACCGTCTCACTGCTTAGT14.761904761904762No Hit
CCATTTTCTAGACTCCATAATAAGTATTCAAATGAGTAAGTGTTTTTTCA14.761904761904762No Hit
CCCGTCTTCAAGCTGCTTACCGGCAAAGATAAGCCTCTGCTGATCCGGAG14.761904761904762No Hit
ACAAAATCGATGAAATGGAAGAAATCCGAGTGAATGGAAAAGACAAAATT14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)