FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786073
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACC2200.028.6361649
TACGGCG2950.022.5430286
GCGACCA3100.020.32244110
ATACGGC3550.018.7329395
CGGCGAC3750.017.7332128
GATACGG4550.016.1543144
CACCGAG3950.015.94925715
CGCGGAA1807.1538256E-515.5562871
TGCCGGC1708.180686E-414.41166649
ACGGCGA4950.014.1413157
CGACCAC4354.0017767E-1113.67806511
GACCACC4354.0017767E-1113.67806512
CGTATGC5250.013.33324145
GAGCACC10700.013.08402270
CCAGTCC4052.7230271E-912.96287370
TGCCGTC3356.127357E-712.53722749
CCACCGA4751.72804E-1012.52622914
CATCGCG3103.5238827E-612.4192740
GCCGTCT3103.5238827E-612.4192750
ACCACCG4852.4374458E-1012.26795613
Produced by FastQC (version 0.11.5)