FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786073
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACC2150.029.3021229
TACGGCG2950.022.5430286
GCGACCA3150.019.9998610
ATACGGC3450.019.275925
CGGCGAC3750.017.7332128
GATACGG4550.016.1543144
CACCGAG4050.015.55544815
TGCCGGC1708.180686E-414.41166649
CGCGGAA1750.001018223614.0006581
ACGGCGA5000.013.9999037
CGACCAC4252.7284841E-1113.99990211
GACCACC4404.7293724E-1113.52263412
CGTATGC5200.013.46144545
CCAGTCC4002.2628228E-913.12490970
GAGCACC10750.013.02316570
TGCCGTC3305.106267E-712.72718549
CCACCGA4751.72804E-1012.52622914
TGATACG6800.012.35333
ACCACCG4852.4374458E-1012.26795613
CATCGCG3154.2141655E-612.22213740
Produced by FastQC (version 0.11.5)