FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAATAACAGATGCACAAGACTTTTGTGGTCGACGAACACTAAAGAGTT11.1494252873563218No Hit
TACACTTTCATTTCTAACAATCAGATTCAGTTATAAAAAACATTATAACG11.1494252873563218No Hit
CATATAAACAGCTACTCTAGGAGTAAAGTTTTAATCTGGTGCTAACGATA11.1494252873563218No Hit
TCAGCTCATTCTTTTTTAAAGAACACTTACACTAATTACACTCCCTATTC11.1494252873563218No Hit
CTACACCATGAAAGCTTTGAGAAGCAAGAAGAATGTTGGTTGGTGTTTTG11.1494252873563218No Hit
CTACAAATAACAAATATGCATATTAGCAATTACCGAAAGTGTCTACGCAT11.1494252873563218No Hit
ATTAAAATAGAGAACTAAAACACGTTTTAACAACACTATTATGTTAGCCT11.1494252873563218No Hit
AGTTTAAAATTCGAAATCTTTCCCACTCACAATGAGCCATGAATCGTTTG11.1494252873563218No Hit
TTAGGCTTATAAGATGCGGTTGCGGTTTAAGTTCTTATACTCAATCATCC11.1494252873563218No Hit
GTTTCGTCGAGTCATAAGAGTAACACTGGTTTTTTCTTTGGTAGTTTCAC11.1494252873563218No Hit
ATTAGTTAGCCTGTACGTCCTAATCTGAAGAACAATAGAAACTGGATATT11.1494252873563218No Hit
CATAAATTACAAAACCCGATAGAGACGAATCCGAATAAGATAACTAAAAT11.1494252873563218No Hit
ATCAACAAAATTTATCGATTAAATATAAAAAATATAATAAACAATATAAT11.1494252873563218No Hit
ATTGTATTTTCATTTAAATAGAGGCAATAATCTCTATGTAAAAAAAGTTG11.1494252873563218No Hit
AAATAGCAGAATTGTGGCTAGGGAAGTATATTATCGACCTAGCTAACTTT11.1494252873563218No Hit
TAATCAACGACATTCTTGATCTCTCCAAGGTTGAATCAGGTACAATATAC11.1494252873563218No Hit
CACATTTCAATTGCATCAAACTACATTGAAGAATATAACTTGTGGACTAC11.1494252873563218No Hit
CCTTGTCTCGCCTAGGTTGGATGGGCGTGCTTCGTTGGAAAGCATGGATC11.1494252873563218No Hit
GATAATAATTAGGTTTTATATGCATACCCTATGAAATCAATAATACTTTA11.1494252873563218No Hit
GCTTAAGCCAGTTGTGGTATAGTGATTTGTGTAATTCTGTTTTATGCATG11.1494252873563218No Hit
ATAACTCTTATAATTAGTCAAAAGAATAAGATTGTGACACATTTTTACCA11.1494252873563218No Hit
CGTTATAGAGACATGAAATTCAATGTGGACAACACCCATCTCATCCAGGT11.1494252873563218No Hit
CAACATCTTGTCCATTGATCTTTTCAACCAGACCATCAATCCAAGCCACA11.1494252873563218No Hit
ATTTAAGGGTTACGATCTATTGTTGTTTGTCATGTAAATAAAGGTTATGT11.1494252873563218No Hit
CCCGGCGGCCGGCCGGTCGATCATGCCACGTGCCGATAACCGAGACCAGC11.1494252873563218No Hit
AGTGATTACGTACTATATAATTAACAGTCATGAAAAGCAAACCGCAAACG11.1494252873563218No Hit
CCTCTTTGTTCCCAAGAGAGCTCCTTTCGATCTCTTTGATACTAAGAAGC11.1494252873563218No Hit
CTAGTGCAGAGAGTCAGATTTACAAAGGAACGATCAAAGGCGGGACTGAG11.1494252873563218No Hit
ACTTTGCATGCTTTTGGAGTTCAAACAGAGGAAAAACAGAACATGAAGTC11.1494252873563218No Hit
AACAGAGAAGAAACAAAAAGTAATGAAGGTTAAAGGACACTAAAGAAGAA11.1494252873563218No Hit
GTCATTCCTTGTGGTGAGATTTATGGGCAAGATCGGAAGAGCACACGTCT11.1494252873563218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTACCCAAAAAAAAAAAAATTAAAAAAAAAAGCGGGATGCAACACGAG11.1494252873563218No Hit
TCATGGATTTGAAAATTATTAAATCTGGAAGTAGAACATATAGCGTCGAG11.1494252873563218No Hit
ATAAGTGGAAGAATCACTTTACCCCAAGGTTGGTTGATTAGTCATCCTGA11.1494252873563218No Hit
ATTGTAAGTTGTCGATTGATTTGTTACGCACCCACAAACGGAAATTTAAA11.1494252873563218No Hit
CACATGCAGATGCAAATGGATTGGTCAAAAACGTCAACTTTGACTTCGCA11.1494252873563218No Hit
TAGGGTTAATTTATTGAGCATGTCAATCCCCTGTAAATTGAGGAAACGAA11.1494252873563218No Hit
GCTTCGACGGTGGATCTGTCTCCTCCGATTTCTCACTACTCCGCCGCATG11.1494252873563218No Hit
TTCTTCTTAGTTTTGTTCCTTGTGTTCTGAAATCTTGTGCCTGTAGATAT11.1494252873563218No Hit
ACCAGATTTGGATAATTTCACAGATATCACTTTTATTGTCCCTGCAAAAT11.1494252873563218No Hit
AAACCAAATGCCTTATTTTTGATACCATCTGTGCAGATATGGTAGGTGAA11.1494252873563218No Hit
TTTATCCTTCTTGTGGGTGATTAGACCTCCACCGCACGATCTAAAGGTCG11.1494252873563218No Hit
GTTTATGGTGACATGTTTGATAGGCCTTTTTAAGTGTGCAAGCACGAGGA11.1494252873563218No Hit
AAGTGTTGAAAGGTAAATTTTGTAGTTTCTATTGGTAAATCGACTCATCT11.1494252873563218No Hit
GGTTTAGACAGCATCAGGCTTTGGTTAGAAGAGAAGATTGTTAAGAAAGA11.1494252873563218No Hit
ATAGTAGGAGAAGGCCGGAGCATGAGCCGAAAATGTAACATCGATCGTTA11.1494252873563218No Hit
GATGATTAACTATTAAATGATCTTTTTTACCGGAAGGATAAGAGATTACC11.1494252873563218No Hit
GTCCACAACATCCTCACCATCTAGAGTAACAATTAAGCGCAGAACACCAT11.1494252873563218No Hit
TTCTTATTCAAATATGTTGAGAATCGACGAGAGACGCGATTAAAATATAG11.1494252873563218No Hit
GGCTTGCTCCATCTACAAGTAGATTCAAGCTCTACGATCAATTCGAGCTT11.1494252873563218No Hit
TTATTCGTGAAGGTTTGGTCCCCTCAATCACTTATGTTAATTCTAGTCTT11.1494252873563218No Hit
CTTCGATAAGAGCTATTGGCATGTTGTGGCGTATCTAGCAAAACGTTTAA11.1494252873563218No Hit
CCAAGTCGAAGATCTTACAATGTTGAATGACAACCACCGAGAGATCTATC11.1494252873563218No Hit
AAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATTGTGTAGC11.1494252873563218No Hit
CTATGATTTAGGTATAGTTTTTAGAGTCCTTTTGTTATTTGTAGAGTGTT11.1494252873563218No Hit
GATCGTAGTCTTTTGGAGTCGATTATGACTTGATCTCAAGTGTATGGGTG11.1494252873563218No Hit
ATTTCTGTCCTAGTTGATCCTGATTCGACAGTAAAAGTATATTGATTTTT11.1494252873563218No Hit
TTCTCAAAGCTTTGTTGGTTTAGACAAAGTCCATATAAGTCTTTGTCTTT11.1494252873563218No Hit
AGCTAATGCAGTAATGGTTGATGCATTTGTAGGGTCTGTAGATTCTCCAA11.1494252873563218No Hit
GCAAGAAGAAGGATGGTTAGTGTTTTGGAGTCGATAAAGACATGAAGTCA11.1494252873563218No Hit
CTTGGCTAAAATAACTTAATTTACAAGACTCAACACTAATAATTTTTTTA11.1494252873563218No Hit
ATGAGACTTTCATCTCGCACGGCTCCTAAGTGATAAAAGTAAAGAAGAAC11.1494252873563218No Hit
CCCCATAAGAATCATATTCTGACTTTTATCGGGAGAATATCCAACTACAG11.1494252873563218No Hit
ATATGAGCACATTACTCATGTTTTTGGGCTGAGAAATAGTGGGTTTTGTT11.1494252873563218No Hit
CAAATGAAACTAGTCAATGAACAAAAAAAAAATGGAAGCAGCTTAAAATG11.1494252873563218No Hit
CTTTATACTTCTTTCCACATACGGAAATATGAAATTCAGACGCATGTGAC11.1494252873563218No Hit
TACATCCTACAATGCTACTAAATCTCCTGAAAATGGGAGAAAGACAAAAG11.1494252873563218No Hit
CTAAGTAGTGTATCCTTGTTAGAAGACACAAAGCCAAAGACTCATATGGA11.1494252873563218No Hit
CCTTAAACGTCAAAATTTGGAGTCGACAAAAAGTCAATGGAAAAGGTCCA11.1494252873563218No Hit
CACCGAGCGAACGTAAATAATTGTAACCATATAAATATAAAATTACAGCA11.1494252873563218No Hit
ATCACGTATGGCCATAATTCAATATTATTTGAAATTCACTACATATTAAA11.1494252873563218No Hit
AATAGATTAGTTTATCTTATGTTATTCTCTAATAATTTTTATTATTTAAC11.1494252873563218No Hit
CCATTCACAGGAAACAATATGACTTGGGTTGGGAAAAGGAAAACTCATTA11.1494252873563218No Hit
AATGGATTCAGGCTTTAATGTTGAGATTTGCAAAGTAAGCCCGGCGTCTT11.1494252873563218No Hit
TAAATTTAAAATCGTTTTGTAGGATTCTTTCTTATTTCGTCTATGTCTTA11.1494252873563218No Hit
GAAGCGACTAAGAATTCGTAAAGATTAAATATATGGCAACCTACCAGGTT11.1494252873563218No Hit
TGTGTCCTTGCACAAGATCAATTGCATCCTTTGTACGGAACCAAGAAGAA11.1494252873563218No Hit
AAAAATTGATGTTTTTAAAAAAAAAAAATCCTATTATGTAAAAATATATA11.1494252873563218No Hit
CTGTGTGCAATGAAAAATGCAATCACGGTTTGGGGAGGGGTTTTTTACCT11.1494252873563218No Hit
TGGAAATATGACACAAAAATGACACACATGCACAAAAGCTTTATAAGATC11.1494252873563218No Hit
CCTATATACATCACAATCGGGGATGGCGGAAACTCGGAAGGCTTGCTTAC11.1494252873563218No Hit
TGTGACCACGTCTCTTTAGAGACAAATTGCTTACGGTAGTTATCGTTTTT11.1494252873563218No Hit
AAATACATCAAATTTGCTGGATGTCAAAATATACATTTTATCAAATTTGC11.1494252873563218No Hit
CGGGCAACAGAATATGCTATATCTTCTACAGATCTTTGCGCTGGGGGATC11.1494252873563218No Hit
ATGTCGGATGGTTGTGTTGATCTGGTCCTTGTATATTAGCAAGTTGAACT11.1494252873563218No Hit
GGTTGCGGTTTAAGTTCTTATACTAAATCATACACATGATATCAAGTCAT11.1494252873563218No Hit
AATGACCTTAGGAGATGCACTCACTCAGGCTAAAGACACAACTCTTGCAT11.1494252873563218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA50.070.020
TTGTCTT50.070.043
TGGTTTA50.070.016
TGTTGGT50.070.013
ACAAAGT50.070.024
TTGTTGG50.070.012
AACAAGG50.070.060
AGCTTTG50.070.08
ATCTTCT50.070.053
ATAAGTC50.070.035
TTGTATC50.070.049
GAAACAC50.070.066
TGTCTTT50.070.044
AAACACT50.070.067
TAAGTCT50.070.036
TATAAGT50.070.034
GTATCTT50.070.051
ACACTAC50.070.069
AGGAAAC50.070.064
GGTTTAG50.070.017