FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_LEHLE_f-2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_LEHLE_f-2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201602
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC3704.3655746E-1115.13502870
AAGAGCC4701.4370016E-1012.65948570
CGTGAAA8050.011.73904770
CGGCAGT2408.1706606E-411.66658352
TAAGGGG2751.9832936E-411.4544632
CACGTGA9600.010.93742270
AGTCACG14150.09.89392270
CGGAAGC4401.605622E-59.54538670
CAGTCAC15650.09.39329569
ACGTGAA10050.09.05503869
TCCAGTC19700.08.70552270
TCACGTG11550.08.48514169
CCAGTCA17050.08.21142768
GTCACGT13650.08.2050770
CTCCAGT22700.08.17174870
ACTCCAG24000.07.72943369
ATAAGGG5057.3769095E-47.62370821
GAACTCC30250.06.942099670
AAACCGC14552.910383E-116.73534733
CCCGATC5950.0041736416.470542442
Produced by FastQC (version 0.11.5)