FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_LEHLE_f-2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_LEHLE_f-2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201602
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC3704.3655746E-1115.13502870
AAGAGCC4601.0004442E-1012.9346970
CGTGAAA8000.011.81241670
CGGCAGT2408.1706606E-411.66658352
TAAGGGG2751.9832936E-411.4544632
CACGTGA9550.010.99468670
AGTCACG14100.09.92900870
CGGAAGC4351.4022984E-59.65510470
CAGTCAC15600.09.42340269
ACGTGAA10000.09.10031469
TCCAGTC19650.08.72767470
TCACGTG11500.08.52203369
CCAGTCA17000.08.23557868
GTCACGT13600.08.23523570
CTCCAGT22700.08.17174870
ATAAGGG5006.6327467E-47.6999451
ACTCCAG24050.07.567828769
GAACTCC30300.06.930643670
AAACCGC14401.6007107E-106.562453333
AACTCCA30450.06.09216468
Produced by FastQC (version 0.11.5)