FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_6911_f-2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_6911_f-2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064576
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCC1101.0168587E-625.45434270
GAGCACC3458.730549E-712.17381570
TCCGCAC4357.863491E-912.06886867
CGCACAT3155.6467856E-511.11102269
CGTCCGC6301.2732926E-1111.11102269
CCGCACA3501.35908995E-510.99991268
ACGTCCG7351.8189894E-1210.47610769
CAGTCAC16100.09.7825370
GTCCGCA4703.3284105E-69.68077366
CGGAAGC4251.14761104E-49.0587570
CCAGTCA17100.09.00577569
CACGTCC9605.475158E-108.02076968
TCCAGTC20800.07.908590370
AGTCACG14350.07.80481569
AAGAGCC5006.630993E-47.699938370
GTCACGT14700.07.618986670
CTCCAGT23150.07.40814869
CAACCGG5854.445526E-47.179429550
GGCGTAA8057.4254312E-66.956465755
TCACGTC14907.2759576E-126.812025570