FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_6911_f-2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_6911_f-2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064576
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCC1101.0168587E-625.45434270
GAGCACC3458.730549E-712.17381570
TCCGCAC4409.315045E-911.93172367
CGCACAT3104.7974878E-511.29023269
CGTCCGC6351.4551915E-1111.02353469
CCGCACA3551.5903093E-510.84498368
ACGTCCG7351.8189894E-1210.47610769
CAGTCAC16000.09.84367170
CGGAAGC4301.2222157E-59.76736470
GTCCGCA4753.8091202E-69.57887166
CCAGTCA17050.09.03218669
CACGTCC9605.475158E-108.02076968
TCCAGTC20750.07.927647670
AAGAGCC5006.630993E-47.699938370
AGTCACG14250.07.61397469
GTCACGT14600.07.43144770
CTCCAGT23100.07.424182469
CAACCGG5854.445526E-47.179429550
GGCGTAA8108.140021E-66.913524655
TCACGTC14857.2759576E-126.83496270
Produced by FastQC (version 0.11.5)