FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_6911_f-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_6911_f-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAAGTTCTTATACTCAATCATACACATGACATCAAGTCATATTCGACT20.12795905310300704No Hit
TTATATGTAACGTGGGAGATGCGACTAGATACAGATTACACTAATCGACG20.12795905310300704No Hit
ATATCGTGGACAGTGCATAATAATTCAAGTGAATTTGGTATATGTTCCTT20.12795905310300704No Hit
CTACGTACAAATAACTGGAAGCAATATGGAAAAGAGATCTGCTTTTACTG20.12795905310300704No Hit
CATCGTTTGCGGCAACATACCTTTTCTTAACCGATCGTAAGTCGGTAATC20.12795905310300704No Hit
AGTTTAAAGCACAGCTGCAAAGTTCCTTCCAGGAGATATCATCACTGGAG20.12795905310300704No Hit
TTTCGTAATTCATCTGTGTGTGTGCCTTTTGCTTTTGTAAGTTTAGCTCA20.12795905310300704No Hit
TTCAAAACAGCTCAATCTCATTATCCACGTGGCAATATCTCCATGGTCAC20.12795905310300704No Hit
ATGACAAATATGAAGTGATAAACTTTAGAACATTTTTTGATAATATCATG20.12795905310300704No Hit
CTGGTTGAAGAGGAAGCATTACCTGCTGAACAAGCGGATGAATTCAAAGT20.12795905310300704No Hit
TATAACAAACTTGATCTGCTTGAGATGCTATATACATATATCAAACAAGA20.12795905310300704No Hit
TATCCATATTTAGATCAATTGAATTCATACTAGGGGAAGGTGATGACTCG20.12795905310300704No Hit
TCGAATATGACTTGATGTCATGTGTATGATTGAGTATAAGAACTTAAACC20.12795905310300704No Hit
TTATTATTTTTGGAAAAACTTTAACAAAATCCTAGGCTAAAGATCGCGTT20.12795905310300704No Hit
AATTATTCAATATGATAACCAACTATGAATTAGCTACATGATTGGATCAT20.12795905310300704No Hit
GCATTCAATGAAAAAACATGTAGAAGATGATGATGCAACATGTGTTTAGA20.12795905310300704No Hit
CTAAAGAGATTGACAGAACATCAACACCATCATGCATAGCCTCGTCCATA20.12795905310300704No Hit
AAGAGATGATGAGAGCAGGAGTTCATTTTGGACATGGTACGAGGAAATGG20.12795905310300704No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers