FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_5784_f-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_5784_f-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309645
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACA2102.652387E-413.33333465
ATATCGG2900.00394949559.65517369
AAGAGCC5151.035618E-69.51456470
TCCCCAC2950.00454800349.49152655
CGGAAGC5007.314331E-69.09999970
ACGAGTG6603.8975486E-89.01515170
GAGCACC3150.0077996878.88888870
AGTCACG11450.08.55895269
CGAGTGG5805.180911E-68.448276569
GTCACGA11250.08.40000170
TCCAGTC16500.08.27272770
CAGTCAC14050.07.971530469
CTCCAGT18600.07.526881769
CCAGTCA14200.07.394366368
CACGAGT8059.419091E-77.391304569
GAGTGGA6201.0818755E-47.3387170
ACTCCAG20650.06.949152569
CAAGAGC5550.0020100666.93693770
TCACGAG9159.592604E-76.88524669
TCTGAAC33200.06.746987369
Produced by FastQC (version 0.11.5)