FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_5784_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_5784_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTAGTTAATGAGTGATCATGGCATGAGATGTAGTATGCACACGTGG15.88235294117647No Hit
GATTTAATTATTATGATTTTATGACGGTACCTCCACGTCAACATAATGGC15.88235294117647No Hit
GCAGTGCGAGAATTGAGCTTAGGAACTACAATCGAGCGTTTTGATTCTCA15.88235294117647No Hit
TTATCTGTGATGAGTTTGTTTGAGGTATGGTATTACACTAATACACTAAA15.88235294117647No Hit
CTCCGGGAAATCAAGCAAAAGAAGCCAGAAATCAGAGCATTTTAATCGAT15.88235294117647No Hit
ATTGATACAGTCGTAAAGGAGATTCTTATCGTCGCAGAGCTGTGTAGAAG15.88235294117647No Hit
TTTCTTTTAACATCAATTGTGTGTTGTTGACATTGTTTCTCAGCCAAAGT15.88235294117647No Hit
TTGATTTAACAATTTTACAAAGGAGATAACCCAAAACCGTAAGTCAATCA15.88235294117647No Hit
CCACATCATTAAATGTAATGCACATTTTGAATGAACAAAATAGGCATCAG15.88235294117647No Hit
GTTTGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAATATGAC15.88235294117647No Hit
ACGATGGGCTGTTTTAGTTGCTGGATCAAATGAATATTATAACTACAGGC15.88235294117647No Hit
GGCCGGCGTTACAATGGGCACTTCACAACATCCTCCTTCAAGGTGACGTC15.88235294117647No Hit
AGGTACTCGACAATGGGGTAGAGTGATTGGTCGCGTGAATGATGATGTGG15.88235294117647No Hit
GGGATCTCATTGAGCATGGAGTTATGCAGCCAGAGAGAAACGTGATCCTG15.88235294117647No Hit
CTGAGATATTGCGATATCAGGTAGGTCTCATACCGCAGAACGAGCCATTT15.88235294117647No Hit
AATCCCATTATCACTTGGTAACTTAAACATTAATAACGAGAAATTATGGC15.88235294117647No Hit
AAAACATCATTAATGAATCAATCTTCTCAACTGATTTAGTTTTATCCTAA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)