FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_1741_f-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_1741_f-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270377
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC2800.021.24997770
AAGAGCC4752.8883733E-811.05261970
CACACTG8350.010.05986970
TGCCGTC3702.8748057E-49.4594492
GCCGTCA3750.0034053018.4006521
TCCAGTC15950.08.11911369
CAGTCAC13900.08.05754569
CCAGTCA15000.07.93332470
CTCCAGT18800.07.6329770
AGTCACA14900.07.28187170
ACTCCAG21150.07.115831469
CGGAAGC5050.0062005126.93068570
GAACTCC25700.06.80933169
TCACACT12552.4610927E-96.693219769
AGGCGTA5800.0031949716.637923763
GTCACAC13251.1423253E-96.603766469
ACACTGA9551.9415893E-66.596851369
GTCTGAA38100.06.52230270
CGTCTGA38700.06.511620569
ACCCGAT8601.9726023E-56.511620552
Produced by FastQC (version 0.11.5)