FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_1741_f-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_1741_f-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270377
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC2800.021.24997770
AAGAGCC4803.3698598E-810.93748970
CACACTG8450.09.94081770
TGCCGTC3753.2891968E-49.3333232
GCCGTCA3800.00383787078.2901171
CAGTCAC14000.08.24999169
TCCAGTC16100.08.04346969
CCAGTCA15100.07.88078670
CTCCAGT18900.07.592583770
AGTCACA15000.07.466657670
CGGAAGC5000.0056301556.999992470
ACTCCAG21250.06.917639369
GAACTCC25700.06.80933170
TCACACT12652.921297E-96.64030969
AGGCGTA5800.0031949716.637923763
GTCACAC13201.0495569E-96.628780469
ACACTGA9652.303661E-66.5284969
ACCCGAT8652.1479816E-56.473981452
GTCTGAA38100.06.430438570
CGTCTGA38800.06.40463269
Produced by FastQC (version 0.11.5)