FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_TGA5_1741_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_TGA5_1741_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCAAAATTTCTCTATATTTACCTTTTCTTTTCTCCTCCTCTCTATCTT15.555555555555555No Hit
ACTATGCTGGGATGATGCGAGCAGAATATGGCACGCACAATCCATGAGCT15.555555555555555No Hit
GCAACACATCAAAGTAGAGACTTCAGTCTCAATCATAAACAGGAAGAGAA15.555555555555555No Hit
ATGCTTAGATGGATGTTGGATGATGATGATGATGATGATAATGATGATGT15.555555555555555No Hit
TCTAACCCCAGCTCACGTTCGCTATTTGTGGGTGAACAATCCAACACTTT15.555555555555555No Hit
AAGAGGTCCAGGGAAGAGGTCAGCAGATGCATAGAGGCGATGAGACTGAT15.555555555555555No Hit
GCAACTCATATGATTGATGACAAACTTGGCTCCCCGTATAGTTAGAGGAG15.555555555555555No Hit
CTTATTCTTATTACTTAATCTTATTCTTATTCGCGCGAGCGCGTTCTGGC15.555555555555555No Hit
AATGATTGAAAACAAGTCCTTCGGATCACAAGAGCCGACATTACTTCGAG15.555555555555555No Hit
AACATTTATCACGAAGAAAAAGAAGTGACTTCATCATTAAAAGAAACAAT15.555555555555555No Hit
TTTAGAGAGATGAACTCTGTTTCTCCGACTAGCTTTTTCAGTAAATCGCC15.555555555555555No Hit
GATTTGAAAGAAAATTTAGAAAAATAATAATTTACATCATATGCAATTTT15.555555555555555No Hit
TTTCTGTTTGTTTTTCTCGAAACTCGTCGCTTACACGTTCTTGTATTGGT15.555555555555555No Hit
GATTAGACGGTACGCTTTTGTAAATGTTACAGAATTAAGATGACATTTAA15.555555555555555No Hit
TACTTTTAAGAAAACTCCAAAAAAGCATAACCATAATGTCATAAAGACAT15.555555555555555No Hit
TAGAAATTACTTCTGTGACGTTGCAGATTTCAACAACAGCAGGTTCTCTG15.555555555555555No Hit
CGGAAAATTCGAATTAAAGTTAGGAAACATCCTTACCGGACCTTAGCCAG15.555555555555555No Hit
CTTCGGTCCAATGCACAAGTCGTTATCTGGTTCATGCTCCTGAACACAAT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)