FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_MGT1_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_MGT1_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8347858
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG9600.014.94798570
TCACTGC28850.014.3154970
CCAGTCC16600.012.439815570
CTGACCC10450.012.39240170
GAGCACC30550.011.57124770
AAGAGCC39350.011.56294970
CTGAACG9000.011.27782970
CCTGGAC9650.011.24364264
CACGTCG10350.010.48313970
CGTCTGC19650.09.97459970
CGGAAGC42400.09.90570570
ACTGACC111350.09.77552770
CACTGAC131750.09.51047970
AGTCACG8951.4551915E-118.99445470
GGGGGCC4703.5047062E-58.93615755
CCAATCG21400.08.83181670
CCCCTCG11450.08.5589431
CTGACCA99950.08.50929470
TGGGGGG6958.234347E-78.0576385
CTGGACG12200.08.03287165
Produced by FastQC (version 0.11.5)