FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_MGT1_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_MGT1_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8347858
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG9800.015.35721470
TCACTGC29350.014.07161470
CCAGTCC16550.013.11184270
CTGAACG8900.012.58432670
CTGACCC10250.011.95127470
AAGAGCC39800.011.87191370
GAGCACC30850.011.68562870
CCTGGAC9250.010.97308864
CACGTCG10000.010.15004570
CGGAAGC42300.010.09460870
CGTCTGC19900.09.84929170
ACTGACC112150.09.67458770
CCAATCG21600.09.56022870
AGTCACG9250.09.45950270
CACTGAC132450.09.40736670
GGGGGCC4502.0990827E-59.3333255
TGGGGGG7201.7553248E-99.2362085
CTGACCA100600.08.62826970
CCCCTCG11405.456968E-127.98244531
GTCACTG179250.07.732252670
Produced by FastQC (version 0.11.5)