FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_MGT1_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_MGT1_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGTGAGTCAAAGTTGTGAGATTCCCAAGCTCTTGAGGTATGTGAC12.1739130434782608No Hit
TTTTGTAGAATGGTAGTGTTTGATAGAAAGGAATGGCGCGGCATGTGCAA12.1739130434782608No Hit
GAATATATTCAATTGTGAAGGCTTGAGGGAATTGACGTTTCTGATCTTTG12.1739130434782608No Hit
TGACCCCGACACTGTTGCGTCCAAAGAGTTAGGAAGTTATGGATACTGCA12.1739130434782608No Hit
CCAAAGCTAAAAACTTGGTTTGCTTTTCGTCAATTTATTAACGGAGCTCC12.1739130434782608No Hit
TTTTGTGCTGTAATTTGTTTTGAGTGTGCGATTTTGCATCAACTCTGTTT12.1739130434782608No Hit
GTAGCGTTGCGACACAAGACACCCCAGAAAGAGGAAAGCAACCCCGAAAA12.1739130434782608No Hit
GATTATGCATATTCATTATAGTCTTCGGGGTGGTTAGTGGTTGTTGTGGT12.1739130434782608No Hit
AATCTTCAGGCCGGCTGATCTGAAACTCAAAACCAAATTACTTCACAAGA12.1739130434782608No Hit
TGGTATATTCTTCGAAGGTTAAAACTTTGAGTGAGTTTGAGTTGTGTTGT12.1739130434782608No Hit
TCCCATTGTTCGAGGACCCCTAGATGCTGGCGGAGTAAAGGCTCGTCCAC12.1739130434782608No Hit
CAAGTTACATTGTTTATAGATCCCAATTAATACAAAGTTTTATTATTCAT12.1739130434782608No Hit
CTAGGTTCATCAGTTTTATTAACTCTAGTAAGAATTTTTTCCATGTATAT12.1739130434782608No Hit
CTAGTGTATCTACAGTCTTGTTGGAGAATGTTGGATCTATGCTAAAACAA12.1739130434782608No Hit
TATATTTTCTTGGAAAATGCGGATTATGACAAAAAATATAGTTCACTAAT12.1739130434782608No Hit
CCCTGAATATTCTCTCTACTCTGTTTAGATATGATTCTGATGTAGTTGGG12.1739130434782608No Hit
CTCACATGCGAAATTATAGAATATCCCCTTTAAGATCAAACAATACCATC12.1739130434782608No Hit
TGGTTGTAGCATTAGCGCTTTTATTTGCGAATCCTTTTGTTTAAGCCTAG12.1739130434782608No Hit
TTAGACTTCAGTTCGCAGCACAGCATCCGCCCACACTCCGTCTCCGGGGA12.1739130434782608No Hit
AAACCCTACTGAGGCCTTTAGGATAAGGTTGCGGTTTAAGTTCTTATACT12.1739130434782608No Hit
CTCTATCATCTATATTCATGATTTACCGATTCGTTGTTGTTCTTCTTCGT12.1739130434782608No Hit
TCATCAAGATTTTGTCTTTTGGAAGGATGTGATTTATAATCTATACAATC12.1739130434782608No Hit
CTTACTGAAATTACAAGATTTTTTCAAATAAATCTAAAAAAATCTTGAAT12.1739130434782608No Hit
TTATGAAAAGTGTCTGAGTATGCAATATTCTCTAATTTTAATTTTGGATA12.1739130434782608No Hit
TTTTTTGTGATAAAAATGTTTCGTTTTTTTGTTGTCTTCTTGAATGCTTA12.1739130434782608No Hit
TACCGCTCCCGAAATCCCCGTCCTTTGTCCAAAAAGCATTCCCATACCCA12.1739130434782608No Hit
TGGCTACAGGGAGAATATTCGGGGATATGTGGATTCCTTCGTTTCCATTT12.1739130434782608No Hit
GGTTTCCTGTTGTCACCCGTTGCTTGAACTTCAGCGTACTTTATCGTTGT12.1739130434782608No Hit
GCTAATAACATTGATTCAATTGGCATTGACATAATAGGAATATTTCGTCT12.1739130434782608No Hit
GAGGTTGGTTAGTGTTTTGGAGTCAAATATGCCTTGATGTCATGTGTATG12.1739130434782608No Hit
GATCACGACGACGTGGATCTTCACAATAAAATCCAAACCCTAAAGTTTCA12.1739130434782608No Hit
TTTTCCACTTCTTACTTCCTCCTCTTTAGCCCATCAGCAAATATGATGAA12.1739130434782608No Hit
AATTCAAACACAAACAATTACTAAATACAGTATCCTATTACACAAGAGTT12.1739130434782608No Hit
CTTTACTTTTTTCCCATTTCTAGGGTTTCTTCAATTTCACTCTCTCTCTT12.1739130434782608No Hit
TGTTAGAAGACACAAAGCCAAAGACTCATATGGACTTTGGCTCCACCATG12.1739130434782608No Hit
AAATTATTTGATGATACCATTAGAAATTACTTTTACTCACCCCCGATTCT12.1739130434782608No Hit
GATGTTCATTAAAACAAATATATATATTGATGAATCTCTGTTGTGGAATA12.1739130434782608No Hit
AAAAGGGAGCCGCCGAATACACCAGCTACACCTAACATGTGAAATGGGTG12.1739130434782608No Hit
ATGACAATGATCATGAACACATATAGAGACATATATATGATTCATTACAT12.1739130434782608No Hit
TAAGAAACCAATGTTTAATTGGTTTAGATTCTTTGTATGTGAATTGAAGA12.1739130434782608No Hit
GTTATACTCAGCATATTCGCTGTTAAGGCCTTGGTAATGCAAGCTTTATT12.1739130434782608No Hit
CAATAATGAGTCGTATACAATAATGATTCATGAATCATATAAGAACGATC12.1739130434782608No Hit
TTTTAGTTAAGAACGAGTCTTGGAAATCCTTAGAGTATCTTTGATCCTTG12.1739130434782608No Hit
TAAAGGCAGTTACTAAAGCATATGGAGAAAAAAAAGAGGTATTGTGATTA12.1739130434782608No Hit
TCTCAATTAACTATTAATTTTTAGATGATATGACATTTATTTTATAATTT12.1739130434782608No Hit
TCAATACTGGAAAGCAAGAGAGTGAAGTTTTTGAATCAGATGGTAACGTT12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)