FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_MGT1_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_MGT1_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233138
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC20250.015.72836670
CGGGGGT3655.7480065E-1014.383635543
TCGGGGG6800.012.353003542
ATCGGGG11950.012.30131741
CACACGG8000.011.37497870
CGGAAGC31800.011.33645670
CCTGGAC8450.011.18348964
CCAGTCC14450.010.89963370
AGTCACG7400.010.87835870
AAGAGCC33450.010.56799170
CTGGACG10300.010.53403365
CACGTCG6754.9112714E-1110.37035170
ACTAGCG6603.619789E-1010.07573970
CCTGGTG13250.08.71696550
CCCTCGA14350.08.53656932
GGGGGCC3750.0034112848.40010155
TCCCTCG16300.08.37421731
TTATCGG29250.08.13673770
GCACACC11751.8189894E-128.04253970
GTCACTA124650.07.69352770
Produced by FastQC (version 0.11.5)