FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_MGT1_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_MGT1_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233138
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT3653.45608E-1115.34254143
GAGCACC20900.015.07174170
TCGGGGG6850.012.77378642
ATCGGGG11600.012.37075141
CCAGTCC14400.011.66664470
CGGAAGC32500.011.30767170
CCTGGAC8450.011.18348864
AGTCACG7400.010.87835670
CTGGACG10300.010.87383965
CACGTCG6855.456968E-1210.72990570
CACACGG7850.010.70061670
AAGAGCC33150.010.6636370
ACTAGCG6452.5975169E-99.76742370
CCTGGTG13350.09.43818350
CCCTCGA14450.09.20413432
TCCCTCG16500.08.90907331
CTGGTGG18100.08.121531551
AGTCCTG16100.08.043406547
GCACACC11801.8189894E-128.00845870
GCCCGGT4000.00609789287.87525651
Produced by FastQC (version 0.11.5)