FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_MGT1_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_MGT1_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGAAGATGAAGAGAGAATCTCATCTTTCGATTCTTGCTCCTAGCTGCA10.9090909090909091No Hit
GTAAAATCGTGAGACTAAGTATTGGTGCTTTAGTCCAAAAGGTAAAATCT10.9090909090909091No Hit
TGACTATCAGATATTTATTATTATTACCTGACAGGTTCTCGTTTGTGCCA10.9090909090909091No Hit
CGATTTGTTTTTTTTTTTCCCGTTTTGAATCTCATCGGAATTTTCCCCCG10.9090909090909091No Hit
CTTTCGTTTTAAGTTATAAACTTATAATATAAGCTTGCCTAACATTTGCA10.9090909090909091No Hit
GTTATGTTGTGCAATGATTTGCTAATGATCTTGTTTAGTCCAATTATATA10.9090909090909091No Hit
GACGCACTCACTGAGTTTGAGATTTAAAGTGAACCGGAAAAATGCAGTCT10.9090909090909091No Hit
TCTGCTACCAAGTTCTTGATATTAAACATTTAACTTCTTAGTTCTTCCCC10.9090909090909091No Hit
CGTATTTAGGTCTGAAAGAAGACAGTGAGACTAATTGTTATGCTAATTTG10.9090909090909091No Hit
TATTCCACTTCAAGTCTATTTAACATGAAACCAACACCCCCAAAAAAACT10.9090909090909091No Hit
AATGAATAAGTTAGAGTGGTTTTAGTCCATGCCAATATTTGCAACCATTC10.9090909090909091No Hit
TTAAAGATTCGAATTCACAAAGTTCTGTTGATCAAACAATGAGATGAATG10.9090909090909091No Hit
TTCCGTTTGAATAAAGGAAGGATCCAAAAGAATCGATCTGTCTTTTAGTT10.9090909090909091No Hit
CCACTGGGATTTTAATTTTTGCATTTGATAAATGTTAAACTTTCAGGTTG10.9090909090909091No Hit
AAGAGAGAAAGGACGAGGTCTTGACCGTCATCTTTTGCCCGAAGGACGGA10.9090909090909091No Hit
TGTAATGTGATTTTCTTTATAATAATGTACTTTACATAACATTAATTAAA10.9090909090909091No Hit
TTTTTTTTATTTTTGAAAATTGGAGAACCACACAAGAGATGGCAATAAAT10.9090909090909091No Hit
TTAATAGTTTTGAAAAGGACCTAAGATACTTTGGATATATACTCATGAAA10.9090909090909091No Hit
TCCTTTTAGGGTATATTCATTTTTCACCAAATTACTTGTTTTTTATATGT10.9090909090909091No Hit
CCATTTCCCCTGTTTCTATGTAGATATTCTCATAATAAAATGGGCGTGAG10.9090909090909091No Hit
AATTCTTTGATTCCAGTTAGTATTAGTAAGAGGGCTCTTGAACTAAGAAA10.9090909090909091No Hit
CTTAAGAGTCTTTGAATTGTGGAATCTGTAACCTTAATGGGACAAAAAAG10.9090909090909091No Hit
TGGAAGGAGACCAGTAGACCCAGAGGCACCACGTGAGCAGGAGATTTTGG10.9090909090909091No Hit
GAGGATCTTGAGTATCTAAAGGTACAAACTTGGAATCTTCATAAAGTGTC10.9090909090909091No Hit
GTTCCCCTTCAGGATGGTATATTGGATTCTGGCCCGGGCCAAGTCATCTC10.9090909090909091No Hit
GGTATTAAACTTCTAAATAAAAAATAGTTTGAATTTTTTTTAGAATTTTT10.9090909090909091No Hit
GGTGAGACAACTAAAAAAGAGACAAAAATAAAAACCTATTGTCGCTTGGT10.9090909090909091No Hit
TCATCACCAATAAATTGGCCCCATTACTTGTCACCATTAGCGATACCCAC10.9090909090909091No Hit
TGTTTATGAATTTTGGAAAAAATATATAGATGAATACTAGAGATTCATGA10.9090909090909091No Hit
ATCGGCTTCTTATTAGTCGTCGTCTGCAACTTTTAGAGCCCAGCAACTCT10.9090909090909091No Hit
CGGAAAAGGGGGGGAAAGCTCTCCGGTCCTGGTTCTCCTGTAGCTGGATC10.9090909090909091No Hit
GGGTAAGAGGATTAATTGGTGTATATTCTTTTGTTAATAGCTTGGAGGAT10.9090909090909091No Hit
TGTAGGTATATACATCTATATAGAGATATATGTTTAAAGCAAACGATACA10.9090909090909091No Hit
GACAATCCACCCAGAGCAGGTCGATGTGAGTCTTATACAACAGTTAATGA10.9090909090909091No Hit
ATATCCCCCGAGCCAAGTGGAGCATGCATGAAGGAGTCAGATCCTTCTTA10.9090909090909091No Hit
CAAGTCTTTTGTGACCTAAACTAGATTAGATTATAAATAAACCACACATT10.9090909090909091No Hit
CATTGACATGAAAAGGTATATTGGATTCCATTAGACTTCTCAACATCAGT10.9090909090909091No Hit
TTGTAGCATTGTCTCCAAATGCTTCGCATGCCTCTCTAGGTCCAGGCTAT10.9090909090909091No Hit
AAGCCAGATAAAATATCCCGACACAGTGGCATCTTGATACCGCCAGGAAG10.9090909090909091No Hit
GGAGGTAGAAGGCCTACGGGTCCTGAACTTCTTTTCCCAGAGAAGAAGCA10.9090909090909091No Hit
ATTGGACATCCTCACAAATCGGATCTCGGATCCTGGATTCTAGATAATAA10.9090909090909091No Hit
ATGGCCTTTGGCTACACCATGAAAGCTGTGATAAGCAAGCAGAAGGTTGG10.9090909090909091No Hit
TAAAAGAAAAAAAGGATTTGTTAGAATATTCTAACAAAAACGACATTCGA10.9090909090909091No Hit
ATTGTGTAAATACCTAATTTTTAACAATAAACGCTTTTCGTCCGCCTCCA10.9090909090909091No Hit
CAATATAGTTGGGTTTTATATCTAACATCTCTGTCGTTAGATCGGAAGAG10.9090909090909091No Hit
GGGAAATGCGACTCTGAATCATTGAACTCTTATGAAAGATCCGATCCACC10.9090909090909091No Hit
ATTAGTCGATGCCTTATAACAACGAAGAAATGGTTTAGGAGCGGACTAAA10.9090909090909091No Hit
TTAACCACATTGCATTGTATTACAAAGTATATTTAGAGTTACTAAAACAA10.9090909090909091No Hit
ACATATATACAAATACTATATATGAAAGAATGTTACTTAAAAATGATATG10.9090909090909091No Hit
TACCCTGCCCCAATATGCCTTGCATCTCCACCTGTTCCTAGACAAGTCTA10.9090909090909091No Hit
CTGACACGTGTTCTCCAATATTCTTATACTCATTGACTCTTACCAAAGTA10.9090909090909091No Hit
TGTTATTTTCTACTATTAAAATAAAACTAAAACCAATAAAAGATATTTCA10.9090909090909091No Hit
AAAGAACCTATGCTGAAGCTACTTCAGAAGCTGTCAAATTATCAGGCCAG10.9090909090909091No Hit
GAATTTGCCTGACACTGTTGCCAATTTTGGGCCTTATAGTATATAAATGA10.9090909090909091No Hit
CTTTTCTCACTTAAGCGGATACTCCAAGTAAAATCAATGCTTTAAGGAAA10.9090909090909091No Hit
ACACGAAGAAGGGTAAGACTAGTAGTATGAAAAGGTTATATATTAATATC10.9090909090909091No Hit
CCCCAGCCAGGTGAGATTTTCTTGAATACAAGATTCGCTTTGTGGCGATT10.9090909090909091No Hit
AAGAATGAAAGAAGAGATTCTTACATGATGGATCGAAGATTTAGGGTTGA10.9090909090909091No Hit
TCTGTCCTTTATTTTTATTTCACTCAGTGCGTCATTAGAGCCACAAACAC10.9090909090909091No Hit
GATGATTCTTTATATAGGAGTACAAGGAAAGGGTAAAACCGTAGAAACAT10.9090909090909091No Hit
ATTTCAGAAGGTCTATGGACTTGATCACAATATCATCACTAAGATCTTTA10.9090909090909091No Hit
AGTACTTTATTAGATAGTTGATTTGTGAATGTACTGGATTTAGAAGGAGA10.9090909090909091No Hit
TGAACTTAAACCGCAACTGGATCTTAAAGGTGTAGGAATTGTATACTTGT10.9090909090909091No Hit
CCCAGGAATCACAAGCCGGATCAGACTCTGCCTCAACTACGCCACCACCA10.9090909090909091No Hit
GTCATGATGACATCATTGTCATTTCTGTATTTGTATGTCTTGTACCATTT10.9090909090909091No Hit
AAGAAGCAAAATCTCTAACAAATCCCTAGAATCTACTACATAAGAGGAAA10.9090909090909091No Hit
GAATATTTCGAACAAATCCTGAAATTCAAAACTGCCCGTTATCCAATAAA10.9090909090909091No Hit
CAGAGCAGAATCAATGGTTCAGATTCAATCTCGATTTCCTCCCTTTCCGA10.9090909090909091No Hit
GAGATAGAAGTGAATATATTGTAGAATCTGGAGCGCGCCCGCCAAAATAA10.9090909090909091No Hit
CTCACGTGCGCAAACCAACCGGAGAGTTTGGGATTGCTTTCAAAGAAACT10.9090909090909091No Hit
TAGTGACGATCTTGTGCCAACCCTTGTATGATCCCGTAGCATAGCCACGA10.9090909090909091No Hit
TAGTAAAAGAATAGAAGATTCTTCCGAATCTTAAATGTTCTGTTACTGAG10.9090909090909091No Hit
GGCGGGTCGCAAAGCTCTTTGGGTGTTGTGGTTTCGTGTCTCTGTCGGAA10.9090909090909091No Hit
ATGAAGAATCTGAATCTGATGGTGAGAACGCAGAAGCGGAAGCAGATGAA10.9090909090909091No Hit
AGTTAATGATTAAGTCTATTAATCAGGATATTCAATTTCATCTGATCTCT10.9090909090909091No Hit
GATCAGTTCGTGAAGCACCCCGCGCAAGGAAAGGTCACCCAGTGCAAAAC10.9090909090909091No Hit
GTGATATTCCGAATCCTCATTACTAATGGAATCGAAAGGATCTATGAATT10.9090909090909091No Hit
TTCTTATATAGGTGTCTATATACATGCACATAAAGCTGTGTAATATGTAA10.9090909090909091No Hit
GAATCAATCGAGCGGACTTCTAAAACTTGTTCTACTTTTGGAAGACCTTG10.9090909090909091No Hit
CTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCG10.9090909090909091No Hit
GTTTGAATAAAGGAAGGATCCAAAAGAATCGATCTTTCTTTTAGTTGTTG10.9090909090909091No Hit
TTGCAGCTATACAGCTGGAATTTGGGAAGCGCTTGCTCGAGGATTGCTTC10.9090909090909091No Hit
TTACTACATTTCTTATCTTTGTGAAAAGCTCTTTAATTTCACTATTCTTT10.9090909090909091No Hit
TCTCTAAACTACACGTATGCCGACATACATAAAAACACAACCATCTATTT10.9090909090909091No Hit
GAATACTTTTGTCTGTAAATACTGCATATTTGATTCCATCCATAAATCGA10.9090909090909091No Hit
GCGTTGGAGGTGCTCATAGGAAGTAAGACTTGATCTTCAAAGCTTGATGA10.9090909090909091No Hit
TGGACTCAGGACAAATCCAATCACATAAACAAAAAAATGAAGAAACAAAT10.9090909090909091No Hit
AGTTGCTAATCAGAGGTGGGCAGGGTTCCTCTCCCCACTCTCAACCTTAA10.9090909090909091No Hit
TATGTGGATTTGTTTCAGATCCTAAAGTCTCTATGATGAATTAGTTTTTA10.9090909090909091No Hit
CCCCACATCTTTTAGGATTCAACCAAATCTTTTAGGAAACAAAACAATAA10.9090909090909091No Hit
CGCCTTGCACGGAAAGCAAGCTATCTTCAAGCATGTCTAATGCATGCTCA10.9090909090909091No Hit
CGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGA10.9090909090909091No Hit
AAGGTACGCCCGGTTATTTAGACCCGGATTATCACCGGTCTTTTCGGTTA10.9090909090909091No Hit
CCTCTGCCAACAGACGACTGAATTCCCCTGGTGTGAGAATGACATTCTCC10.9090909090909091No Hit
TTCTAATGAATTTCTATCGAGAGAAACCAGAAACAAATACCTCGGAGAAA10.9090909090909091No Hit
AGATGATGATATTACGTTACGTTATTTTTAAATAAATAGAATGAATCTTG10.9090909090909091No Hit
GCTTAGGCTCTTAAGATCGAGTTGCGGTTTAAGTTCTTATACTCAATCCT10.9090909090909091No Hit
GCCAAACTTGGCATGTGATACCTTTTCGGAAAGCCCAAAGAGAGCCCTCC10.9090909090909091No Hit
ACCGGTGTTAGGATCTCAGGTTCTGGAATCTCATTCGCCGTCTTCTTCTT10.9090909090909091No Hit
TGTTTTTAATTGATGAAAAAAAAACAACTTTCAAGGATTCTTCTTTGCTT10.9090909090909091No Hit
CTTCATGTGGCTCAGTTGCCTTCGTACTTCTTCCGGTAACTGCAATGTGA10.9090909090909091No Hit
GACATGGCCCATTACGGTTACAACTGGAGGCCGTGGAAGAATCTCAGATC10.9090909090909091No Hit
TTTGCGAGCCTGTGCTGGGCTATATCCTCCACCGAATGTATCAGGAGAGA10.9090909090909091No Hit
GCCATTGCCTATGCGGTGAATCGCTTGTCACAATATATGCACTGTCCAAC10.9090909090909091No Hit
CGCGTTACCTCTACCGTTTTACACATCCAAGATGGCTTGGAACGGATAAG10.9090909090909091No Hit
TTCGTCCAGGATTCCTCCACCAGGAGTTGAAATCGTCGAGGGATAAAAAT10.9090909090909091No Hit
CTTATTACTACAGCCAGTCAGCCACGCTTCAAGCATACAATGATCAAACC10.9090909090909091No Hit
TGCATTTGTCAGCACATCTATCAGAAGAATCCTGGATCCTCTGGTTGAAA10.9090909090909091No Hit
TCTTTCCCCGCTGATTCTGCCAAGCCCGTTCCCTAGATCTGAAGAGCACA10.9090909090909091No Hit
AAATGTGATAGGATATACCTCCGACAGAGATCACCTTCTTGGGTGCAGTC10.9090909090909091No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers