FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_LHY1_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_LHY1_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730615
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC10200.014.75512270
TGGGGGG5602.6611815E-910.6250375
CGGAAGC14000.010.00014970
CCGATGT27400.09.58043570
CCAGTCC7101.3624231E-99.36633670
ACCGATG33800.08.4912570
CACGTCG4200.00101927248.33345870
CCCGACT3900.0048588538.07695149
ATCGGGG5804.7374284E-57.844944570
CACCGAT38000.07.644850769
CAGTCAC62850.07.629389370
GTCACCG44400.07.567680469
ATGGGGG4650.00277872947.5269084
AGTCACC53450.07.26858470
TCCAGTC75700.07.259024670
CGATGTA29100.07.21660269
TCACCGA47450.06.85995570
CTCCAGT85650.06.62006469
CCAGTCA70550.06.548644569
ACTCCAG98350.06.334614370
Produced by FastQC (version 0.11.5)