FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_LHY1_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_LHY1_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730615
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC10250.014.68313870
TGGGGGG5904.7293724E-1111.2712185
CCAGTCC7101.2732926E-1110.35226270
CGGAAGC14100.09.43275870
CCGATGT27350.09.34199870
CACGTCG3854.286584E-49.0910470
ATCGGGG5805.1938987E-68.44839770
ACCGATG33350.08.29097469
CCCGACT3800.0038448768.28954549
GTCACCG43900.07.89305269
CACCGAT37300.07.788315370
CAGTCAC62300.07.69673970
AGTCACC53150.07.44131170
ATGGGGG4750.00342159287.36844254
TCCAGTC75200.07.307285370
TCACCGA47000.07.223507470
CGATGTA28900.07.02432269
CTCCAGT84950.06.63340969
CCAGTCA69500.06.54685669
ACTCCAG98350.06.37019870
Produced by FastQC (version 0.11.5)