FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_LHY1_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_LHY1_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951402
Sequences flagged as poor quality0
Sequence length76
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1801.7059392E-719.4441665
TGGGGGG3151.8579158E-814.4442395
GAGCACC4451.6590384E-610.22467770
AAGAGCC7109.59904E-67.39433670
ACGTCTG27900.06.77416570
GAACTCC18500.06.62159470
CAGTCAC11107.5371645E-86.621593570
CCAGTCA12351.2487362E-86.518191369
GCACACG40100.06.28426369
CACACGT40400.06.23759870
CACGTCT34100.06.15833270
CGTCTGA25750.06.116479470
ACACGTC36300.05.88151869
TCCAGTC12557.5751996E-75.856549368
GGAAGAG81700.05.82616970
CTCCAGT13253.3762444E-75.811296570
TAGATCG51400.05.787913370
CGGAAGA80250.05.62614569
AGCACAC45650.05.520239468
GTCACAC10152.18658E-45.51721870
Produced by FastQC (version 0.11.5)