FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_LHY1_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_LHY1_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951402
Sequences flagged as poor quality0
Sequence length76
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1702.1229516E-618.5291485
TGGGGGG3252.802335E-813.99985
TAGGGGG2550.001358395210.9802354
GAGCACC4502.0986043E-59.33329570
AAGAGCC7301.4193187E-57.1917570
ACGTCTG28450.06.643205670
GAACTCC18500.06.62159470
CAGTCAC11208.93524E-86.562472370
CCAGTCA12401.357148E-86.49190869
GCACACG40750.06.269912269
CACACGT40800.06.26222970
CTCCAGT13305.707807E-86.052605670
CACGTCT34850.06.025799870
CGTCTGA26500.05.943371370
ACACGTC36700.05.817414869
TCCAGTC12709.445994E-75.787377468
TAGATCG51950.05.726636470
GGAAGAG82000.05.71948870
CGGAAGA80700.05.59477269
TCGGAAG83950.05.503252568
Produced by FastQC (version 0.11.5)