FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_LHY1_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_LHY1_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATTCAATCAGCTTTATTTTTGATCAAAAAAAAAAAAAAAAATATAT11.7241379310344827No Hit
GGGCGTAAAAATAAATAATCAGATTTTTTTTTTTTGAGCAAAAAACCACA11.7241379310344827No Hit
TACAAGAGAATTATCTTTCTGTTTCCTTATATATGTATATATGTATCTCC11.7241379310344827No Hit
GGCTTTTGTTCAGCCAGAAATGTTTTATTTTTTTGGGTTCAATTTAGAAA11.7241379310344827No Hit
GATATTTAATCAGATATTTAACTTTTGTGCTAGATTGTTTGAACAAAATA11.7241379310344827No Hit
CTCAAAACGCTCTGACTTTGAAGGCTAAAACACTTATCTTTACCTTTCTA11.7241379310344827No Hit
TATATGCATGCATGGATGTAATCCACTAACTTTAAATTAGCAAAGATGAA11.7241379310344827No Hit
GGAATATTTTTTAGATCTTAAAAATAGCGGATAAAATCTCTAATATTCTT11.7241379310344827No Hit
TACTCCTACGAAGTATATGATGCGTGACTCCAATCAAAATCTTTGACTTG11.7241379310344827No Hit
AGAATCTCAAGGATCAAGGACGGCCAAATAGTCTTGTCTTCAATAATTTG11.7241379310344827No Hit
CCCCAAAATATCTAAACGAGACTCTCTTTCGACTTTATCGAGCTGCCTAA11.7241379310344827No Hit
ACACACACTTGCAAATACGCGACTTTAGGGCAGATAAAAAATCCAAACAA11.7241379310344827No Hit
TTAAAACATATAGATTTTACTTAATATAAATTAAATAAACCGTCGCCGCT11.7241379310344827No Hit
ATAATATATAGAGTTTAAAAAAATCTTAATTTTTTCCGTTATATTTTATA11.7241379310344827No Hit
TATCTCCGGTATATTACCTACAAAAATATCAAGCTCGCGAAGAACGAACA11.7241379310344827No Hit
CTGGCTTAGATTGTATGTTGAATTTAGCCGATAAATTTCTTTGATCAAGG11.7241379310344827No Hit
AGTGTAGAGAGGTAACTAGTACGTGTAGAGTCAAGATTTAACATACTCAA11.7241379310344827No Hit
GATAATATCTGAGAATATTTAAACAAACTATCTTATTTTTTGTGTCGATC11.7241379310344827No Hit
TTTGTTAGGTATTGATAAAAAAAATATATGAAAATATCTTATAAGTTTAT11.7241379310344827No Hit
ATCAGATTAATCTAAAAAATTATTATCAAGATATAAATTAAAAAAAATAT11.7241379310344827No Hit
ACATCCTGCCACACTCCATGAAAGATCTAAAAGCTATGGTATCATTAGAA11.7241379310344827No Hit
AAGAGCTGCCACACAATATTTTTCTGAAGCTATTAGTCAGAAATATAAGA11.7241379310344827No Hit
ATCTGAAGTAATCAATATCGGTCAAAATATCTATCTTTTGACATAAGTCG11.7241379310344827No Hit
TTCAAAAAACCTAATCTATATCATAAGTTCAAGTTTCTTTCATTGTCATG11.7241379310344827No Hit
AAATCCTATTGTAACTTGTTATGTCTTTCACTTTAAACAAAGAGATCCAA11.7241379310344827No Hit
CTTATCAAGTAAGTAACAATGATATAACAAATATCTCTTTCTATATATTA11.7241379310344827No Hit
GAGCCAAAAAATAGAAATAGATATTTAACCCTTTGTCCAAAAAAAAGAAA11.7241379310344827No Hit
CCAATCATGGCCCTTGCGGATCGGATCATCCATATAATATACAAAAAGAA11.7241379310344827No Hit
AAGATCAAAAATATGTAACAGATTCTTGTCTTAAGGATTCTTTTTTGAAA11.7241379310344827No Hit
GAATATCTCGGGATTTAATCCCATAGAGAGGAGAAATCTTTCGCTGGTAA11.7241379310344827No Hit
ACCGCAAAGATATTTGATAGCATTTAATTACTACAAAATTACAAAATATT11.7241379310344827No Hit
GGTTCCGCTTCTAGGTTTTATACTCAGTCATAGACATGACATCTAGTCAT11.7241379310344827No Hit
ATCCCCGACGCATCCACTTTCAATGTACTATAACGTGATAAACAAGAGAT11.7241379310344827No Hit
GTTTTCGTCTAACGAGACTAAAGAAGATATTAGCACCAACAATCTCAAAT11.7241379310344827No Hit
AATACTACTACAAATTGTTCTTACATAGTAAAGATATAAGTTCATACAAA11.7241379310344827No Hit
TTATGTGTTTTGGTCAAAAATATCGCCTTTTTGTAAGATGTCTTTTGTGA11.7241379310344827No Hit
TGTTGCTATTATGTTGAATAATCTTGTGTTCTGAAATTCACACTCGAGAA11.7241379310344827No Hit
TTGATTAAAGATATTTATATTTGAAGAAGAAGAAGGAAAGGGACAAAGTT11.7241379310344827No Hit
CCATAAACTATCTAGCCCAACATGTCATGTTAAATTGTTAATTGTTAGGA11.7241379310344827No Hit
TTTCTAATGACATCCATATATAATATCTTAAAAAAGTAAGGATATTTTTA11.7241379310344827No Hit
AACTGTAATTTTAATTATTCTAGCTAAAAACTAAAGATATAACAATTATT11.7241379310344827No Hit
AAAAAATGAGTGAAACTGTAATGGAATTTTTCACCAGCAGAAATATAGTG11.7241379310344827No Hit
AATACATAAAATATTTAATTTATTTTCTATTACAATTATCTAGATATTTT11.7241379310344827No Hit
GTGGACGGATTCCCCTCCGTTTATACAAATGTGTTGACAAAGTCTGTTCT11.7241379310344827No Hit
TAGTGGTTAACTTTCAACAATGTTTCTTAATTGCTAAGATGTTTAACACG11.7241379310344827No Hit
TATAAACTCACATGGACATTTCAGAACACCTGGCTTCGAATTTAATTTTA11.7241379310344827No Hit
TCATTAAAGGACTTATTCCCCCCTATGTTTGTGTTCGCTGTGTCTTTGTA11.7241379310344827No Hit
AGCTCTTGTAGTAGATTATTTAGTTCGTCACTGATTCTCTTACCAATTTC11.7241379310344827No Hit
AAAGATATTTTTATAAATATTTTATGGTAAAAGACGTTTTTGAAAAAAGT11.7241379310344827No Hit
AAATAAATAATTTTACATATATATAATTTGAATTAAAATAGCCTGATATG11.7241379310344827No Hit
AGTTTACACATATTTAAAATTTAACACTTAAAAACCAAAAGATTATGTAT11.7241379310344827No Hit
ACAATATCAAAGATATTGCAGATATAAAGAAGTAAAATAAGAGATGACTT11.7241379310344827No Hit
GTATTTAACCTTTAAAATACATTGTAAAACATTTCCTTTCAAATAGCTTT11.7241379310344827No Hit
ACTTAGTATAATTATATAATAGATATAATAGAAATAATAAAAATACAAGA11.7241379310344827No Hit
TGTTTTCAAAGTTCATTTCATTACAACACCTAAATATAAATACAACTATA11.7241379310344827No Hit
AAAAGTTCATTATATCCTTTTCTCAAACAGTTCTATTTTTTCTTGTAGTC11.7241379310344827No Hit
TTTAACTTCCTTAGAAGATGATATACATATAAGGAAGATTTGAATCTCTA11.7241379310344827No Hit
TGATGTACTGTCTTTCTTTCCTGCGTCAGTTCCAACTCTTTGGTAAGATT11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATT50.070.011
GGTGATC50.070.061
CGTCACT50.070.026
CCAATTT50.070.043
GTCACTG50.070.027
TTCATAT50.070.048
AGCTCTT50.070.01
ACTGATT50.070.030
TTGTAGT50.070.06
TTCGTCA50.070.024
TTACCAA50.070.040
AATTTCA50.070.045
AGTAGAT50.070.010
TTATTTA50.070.016
CTCTTGT50.070.03
TGATTCT50.070.032
TGATCAG50.070.063
CTCTTAC50.070.037
GAACGGT50.070.057
TTTCATA50.070.047
Produced by FastQC (version 0.11.5)