FastQCFastQC Report
Sat 3 Jun 2023
HG2FFAFX5_n02_AbvTbe2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2FFAFX5_n02_AbvTbe2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7784280
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2823963.6277728961445375No Hit
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG709210.911079765887147No Hit
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG664210.8532709511990832No Hit
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT484470.6223696989316931No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG461740.5931698243125889No Hit
CCTGGGATTTGTGGAAGGCGGAGAGTCAGTTCGCGGTACTGGAGGAGGCG393570.5055958932618045No Hit
CCTGCCATCCAGTTCCCGCGAAGCTGGTCTTCAGCCGCCCAGGTCTTTTT347300.44615558535921107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)