FastQCFastQC Report
Sat 3 Jun 2023
HG2FFAFX5_n01_AbvTbe2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2FFAFX5_n01_AbvTbe2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7784280
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3115854.0027465610178465TruSeq Adapter, Index 9 (100% over 50bp)
CCTAGGGCCTCGCAGACCCATCACGGGTCCAGATCCCGGTCTTTTCGCAG728760.9361944842682944No Hit
CCTCTGCAGTCGAGCCAGAACCCTGTTTGAATTCACTTCCGGCGTGAATG703720.9040270904952031No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG495910.6370659842657252No Hit
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT481760.6188883236471453No Hit
CCTGCCATCCAGTTCCCGCGAAGCTGGTCTTCAGCCGCCCAGGTCTTTTT397190.5102462912433776No Hit
CCTGGGATTTGTGGAAGGCGGAGAGTCAGTTCGCGGTACTGGAGGAGGCG374480.4810721094308016No Hit
CCTGAAGTCCGGCTGGAGTGAGTGGGAAGAGAGCGCCACGGACAGTATGT80720.10369616714712214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)