FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8632380
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG2537262.939235761168994No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAG2524092.923979250218364No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGA2377182.75379443444334No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGG2346982.7188098763029434No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT778160.9014431709447452No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC759220.8795025242169598No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT756760.8766527886863184No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC735580.8521172608249404No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTTG176610.20459015937667246No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTT171220.19834622664896584No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGT169030.19580926696924836No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTT161410.18698203739872435No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG94600.10958739073117726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)