FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_Donor3_top10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_Donor3_top10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4168711
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT52359212.56004553925662No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC50784112.182206921995792No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC48278511.581157820726839No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT45842110.99670857490481No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGA2658976.378398502558705No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAG2564586.151973595675018No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGG2431485.832690248856301No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG2264175.431343165789137No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGTAGGCGCGCCGC690261.6558115926002066No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGTAGGCGCGCCGCT672221.6125368249322154No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGTAGGCGCGCCG649661.5584193771168113No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGTAGGCGCGCC610481.4644334903522935No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTTG214710.5150512952325071No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTTGC208400.499914721840876No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTT197980.4749189857488322No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTT181720.43591412309464483No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTT123290.2957508927819655No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTTA117740.28243742490184615No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCT112740.27044330969453145No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGC108710.2607760528374358No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT103190.24753454964856042No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTT101160.24266493887439067No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTTG98700.23676383419239186No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTT97960.23498870514170925No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGT86060.2064427109483003No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAGT51140.12267581034041458No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)