FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_Donor3_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_Donor3_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8241522
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG138890716.85255466162682No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAG133074316.14681123219716No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGG121624614.75754114349267No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGA106217012.88803208921847No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGC240550.29187569965838833No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTTA228670.2774608864721832No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGT224460.27235260671511885No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTTG215680.2616992346801962No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCT212070.25731897579112206No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTT196820.23881511206303882No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT191020.23177757700580062No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC185980.2256622017146833No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTT183460.22260451406912463No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC172660.20950013844530171No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTT169700.2059085688298836No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT150880.18307298093725893No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTT126780.15383080940632082No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTTGC119130.14454854333944628No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTT109140.1324269958874101No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG102330.1241639590357218No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTTG95430.11579171905383497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)