FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_Donor3_bot10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_Donor3_bot10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6510291
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAG91200314.008636480304798No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGA91078813.989973720068734No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG86169213.235844603566877No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGG83473712.821807811663104No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTTG2859514.392292141779838No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTTT2838764.360419526561869No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGT2701664.149829861675922No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAAGAGAGTGTT2640584.056009170711416No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTT1240851.9059823900344854No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCTTA1238371.9021730365048197No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGC1188561.825663399685206No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGATCGTTTCCGCT1141911.7540076165566176No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTTG709221.0893829477054098No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTTGC704451.0820560862793998No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTT661641.016298657003197No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAGAGAGTGTTT652741.0026279931265747No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC280200.4303955076662471No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT277660.4264939923576381No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT265910.40844564398119837No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC257620.39571195819050176No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAGT179740.2760859691218104No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGCATCGGCTACGCG86830.13337345442776674No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGCATCGGCTACGC84520.12982522593844115No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGCATCGGCTAC79610.12228332036156296No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGCATCGGCTACG78080.11993319499850315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)