FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_Donor2_top10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_Donor2_top10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13065136
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGA204727415.669748864458816No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAG202543015.50255580959892No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG200032215.310380236378709No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGG191967914.693142115015107No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT8636866.610616223206556No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC8530016.528833683782549No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT8401226.430258360877376No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC8110346.207620035489872No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAGT412470.31570279865437295No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT175690.13447238513246246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)