FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_Donor2_bot10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_Donor2_bot10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7752230
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAAGAGCAGT129070.16649402816995884No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAAGAGCAGTGCA124290.16032806044196315No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAG120050.15485866647403393No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAAGAGCAGTG117290.15129840058924982No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGAG115490.14897648805569494No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGG112050.1445390552137901No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT95580.12329355553176312No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAAGAGCAGTGC94160.12146182453306985No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC91310.11778546302160797No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC87740.11318033649672418No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAAAAGTATCAGGA86760.1119161841173443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)