FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97143
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2530726.051285218698204No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1608516.55806388520017No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1424614.664978433855245No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC78898.121017469091957No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC64106.598519708059253No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA60516.2289614279979No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG43724.50058161679174No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT29203.005877932532452No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG24832.5560256529034517No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC13611.4010273514303655No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC10991.1313218657031388No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA10251.05514550713896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)