FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115865
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2258819.49510205843007No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1574813.591679972381652No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1377811.891425365727356No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA1362511.759375134855219No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1272010.978293703879514No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1229010.607172139990507No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG37623.2468821473266303No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC26992.329435118456825No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA23011.9859319035083933No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT22991.9842057566996072No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC22511.9427782332887413No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG21071.8184956630561429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)