FastQCFastQC Report
Wed 24 May 2023
HG2CHAFX5_n01_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CHAFX5_n01_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175575
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2707415.420190801651716No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT172499.824291613270681No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC163169.292894774312972No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT157348.961412501779867No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG149918.538231524989321No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA137367.82343727751673No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC132457.543784707390004No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA121786.936067207745977No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC67743.8581802648440835No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG45952.617115192937491No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT30071.7126584080877119No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAC28141.6027338744126443No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT27451.5634344297308842No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG25621.4592054677488253No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA23631.3458635910579524No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC22991.309411932222697No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA20191.1499359248184535No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC11360.6470169443257867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)