FastQCFastQC Report
Tue 27 Nov 2018
HG2CCAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CCAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21377853
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1834150.8579673552811875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGC8950.038.8747331
CGACCCG9550.034.164196145
GGGCCAC10850.031.3991151
GCGCGGC13300.027.795071
GACCTGA13850.026.6912981
GGGCATG10150.025.709031
GCCCGAC5550.024.8147491
GGCAGGT11750.024.6758081
GCCCTGA13800.023.1112171
GAGTTGC14850.021.9652081
GTCTCGG7600.021.9363081
GACGCTA12900.021.9141271
GAGACGG9400.021.5913311
GCGAGTA9450.021.4770931
GGCAAGG16800.021.1415121
GAGCGGC10800.020.8059331
GTGCGGT10250.020.5080031
GACGTGA9200.020.4849451
GCCCTAA13450.020.4790861
GGGCCGT12200.020.200791
Produced by FastQC (version 0.11.5)