FastQCFastQC Report
Tue 27 Nov 2018
HG2CCAFXY_n01_mcb-s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CCAFXY_n01_mcb-s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80191
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2180.271850955842925No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1020.1271963187888915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG1209.449459E-636.25281145
TGGGGGG1306.4512494E-427.8693791
CCCCCCC1305.4198088E-511.15192650-54
AAGGGGG3101.9848874E-57.0166726135-139
AAAGGGG3150.00129125925.9845905130-134
AGGGGGG3404.9528014E-45.971051135-139
CTTCTGC4150.00107641255.24137140-144
Produced by FastQC (version 0.11.5)